相关实验视频

Updated: Jun 29, 2025

Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons

Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons

Published on: August 6, 2014

在现场对mRNA转录的成像,基于活细胞中的分裂和分裂蛋白的成像

Yan Peng^1,2, Mengqi Li¹, Fuyu Gong^1,2

¹The Affiliated First Hospital of Fuyang Normal University, Fuyang Normal University Fuyang Anhui 236037 P.R. China pengyan@fynu.edu.cn gong2022@fynu.edu.cn.

|March 28, 2024

概括

研究人员开发了一种新的双色球探测器,用于检测活细胞中的信使RNA (mRNA). 这种创新的分裂体和分裂光蛋白方法使得精确的mRNA成像具有高选择性和稳定性.

科学领域:

分子生物学分子生物学
细胞生物学细胞生物学
生物技术是生物技术.

背景情况:

使者RNA (mRNA) 在细胞发育和功能中起着至关重要的作用.
目前在活细胞中检测内源mRNA的方法有限,阻碍了研究.
需要先进的工具来可视化生物系统内的mRNA动态.

研究的目的:

开发一种新的,高度特定的探针,用于检测活细胞中的内源mRNA.
为增强mRNA可视化创建双色成像系统.
为mRNA检测和分析建立一个稳定和多功能平台.

主要方法:

开发了分裂体和分裂光蛋白探针.
为特定的mRNA向设计具有互补序列的探针的设计.
使用分裂蛋白启动光补充 (sAPiFC) 进行信号生成.
在活细胞成像中应用sAPiFC方法.

主要成果:

sAPiFC 探针对目标mRNAs 具有很高的选择性.
开发的探针在活细胞中表现出极好的稳定性.
该系统证明能够准和成像各种不同的mRNAs.
成功实现了mRNA的双色成像.

结论:

新的sAPiFC方法为活细胞mRNA成像提供了一个强大的工具.
这种方法克服了现有的mRNA检测技术的局限性.
该技术为研究基因表达和细胞过程提供了巨大的潜力.

更多相关视频

Visualization and Analysis of mRNA Molecules Using Fluorescence In Situ Hybridization in Saccharomyces cerevisiae

Visualization and Analysis of mRNA Molecules Using Fluorescence In Situ Hybridization in Saccharomyces cerevisiae

Published on: June 14, 2013

An In Vitro Single-Molecule Imaging Assay for the Analysis of Cap-Dependent Translation Kinetics

An In Vitro Single-Molecule Imaging Assay for the Analysis of Cap-Dependent Translation Kinetics

Published on: September 15, 2020

相关概念视频

In-situ Hybridization

In-situ Hybridization

In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...

Ribosome Profiling

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...