相关实验视频

Updated: Jun 29, 2025

Interactome-Seq: A Protocol for Domainome Library Construction, Validation and Selection by Phage Display and Next Generation Sequencing
12:04

Interactome-Seq: A Protocol for Domainome Library Construction, Validation and Selection by Phage Display and Next Generation Sequencing

Published on: October 3, 2018

8.9K

作为捕获域-动机和域-域互动的来源,对人类互动组的全蛋白质组评估

Sobia Idrees1,2, Keshav Raj Paudel2

  • 1School of Biotechnology and Biomolecular Sciences University of New South Wales Sydney New South Wales Australia.

Journal of cell communication and signaling
|March 28, 2024
PubMed
概括

这项研究比较了高通量方法和PPI数据库,用于预测蛋白质-蛋白质相互作用 (PPI),特别是域-动机 (DMI) 和域-域相互作用 (DDI). 酵母两杂交 (Y2H) 和AP-MS方法在DMI/DDI预测方面被证明是可靠的.

关键词:
这就是DDDIs的意义.这就是为什么DMI是DMI.个人隐私项目是个人隐私项目.他们是SLIMs.高通量方法的高通量方法蛋白质组是一种蛋白质组.

更多相关视频

Genome-wide Protein-protein Interaction Screening by Protein-fragment Complementation Assay PCA in Living Cells08:38

Genome-wide Protein-protein Interaction Screening by Protein-fragment Complementation Assay PCA in Living Cells

Published on: March 3, 2015

13.3K
Label-Free Immunoprecipitation Mass Spectrometry Workflow for Large-scale Nuclear Interactome Profiling11:19

Label-Free Immunoprecipitation Mass Spectrometry Workflow for Large-scale Nuclear Interactome Profiling

Published on: November 17, 2019

16.1K

相关概念视频

Protein-protein Interfaces02:04

Protein-protein Interfaces

Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a...
12.5K
Protein Networks02:26

Protein Networks

An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
3.9K
Protein-Protein Interfaces02:04

Protein-Protein Interfaces

3.8K
Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
7.3K
Conservation of Protein Domains Over Different Proteins02:26

Conservation of Protein Domains Over Different Proteins

Protein domains are small structurally independent units that are part of a single amino acid chain.  Although these domains are often structurally independent, they may rely on synergistic effects to perform their functions as part of a larger protein. Protein domains may be conserved within the same organism, as well as across different organisms.
A limited set of protein domains often duplicate and recombine during evolution. These domains can be organized in different combinations to...
10.8K
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

1.8K