相关实验视频

Updated: Jun 29, 2025

Bronchoalveolar Lavage Exosomes in Lipopolysaccharide-induced Septic Lung Injury

Bronchoalveolar Lavage Exosomes in Lipopolysaccharide-induced Septic Lung Injury

Published on: May 21, 2018

LncRNA HOTTIP通过招募DNMT1来表观遗传调节SP-C来促进LPS诱导的肺上皮细胞损伤

Shuang Li¹, Shuangjia Li¹, Zhanqun Gao¹

¹Department of Emergency The First Affiliated Hospital of Jiamusi University Jiamusi China.

Journal of cell communication and signaling

|March 28, 2024

概括

长非编码RNAHOTTIP通过DNMT1招募通过表观遗传沉默SP-C促进急性肺损伤. 针对HOTTIP可能为肺上皮细胞损伤提供治疗策略.

科学领域:

分子生物学分子生物学
细胞生物学细胞生物学
遗传学遗传学是一种遗传学.

背景情况:

急性肺损伤 (ALI) 是一种严重的疾病,治疗选择有限.
长非编码RNAs (lncRNAs) 越来越多地被认为是它们在细胞过程和疾病发病过程中的角色.
了解ALI背后的分子机制对于开发有效治疗方法至关重要.

研究的目的:

研究 lncRNA HOTTIP 在急性肺损伤 (ALI) 中的作用.
阐明HOTTIP影响ALI的潜在分子机制.
探索HOTTIP作为ALI的潜在治疗点.

主要方法:

定量实时PCR (qRT-PCR) 和西部斑点测试用于评估基因和蛋白质表达.
细胞计数套件-8 (CCK-8) 测试细胞活力和TUNEL染色用于细胞亡量化.
酶相关免疫吸收试验 (ELISA) 检测炎症因素,甲基化特定PCR (MSP) 检测DNA甲基化,以及RNA免疫沉试验 (RIP) 检测分子相互作用.

主要成果:

脂聚糖 (LPS) 在膜上皮细胞II型 (AECII) 中调高HOTTIP和调低SP-C.
在AEC II细胞中,HOTTIP knockdown降低了LPS诱导的亡和炎症性细胞因子分泌 (TNF-α,IL-1β,IL-6).
霍蒂普将DNA甲基转移酶1 (DNMT1) 引入了SP-C促进体,导致SP-C基因沉默并加剧LPS诱导的肺上皮细胞损伤.

结论:

霍蒂普通过招募DNMT1来表观遗传抑制SP-C表达,从而促进LPS诱导的肺上皮细胞损伤.
抑制SP-C逆转了HOTTIP或DNMT1敲击对LPS诱导的ALI的保护作用.
针对HOTTIP是一个有前途的急性肺损伤治疗策略.

关键词:

在 DNMT1 中,DNMT1 是霍蒂普 (HOTTIP) 是一个热门的酒店.在SP-C中,SP-C是指SP-C.肺部急性肺损伤是什么在ncRNA中,我们可以

更多相关视频

Inducing Acute Lung Injury in Mice by Direct Intratracheal Lipopolysaccharide Instillation

Inducing Acute Lung Injury in Mice by Direct Intratracheal Lipopolysaccharide Instillation

Published on: July 6, 2019

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA

Published on: April 10, 2018

相关概念视频

lncRNA - Long Non-coding RNAs

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...

Non-LTR Retrotransposons

Non-LTR Retrotransposons

As the name suggests, non-LTR retrotransposons lack the long terminal repeats characteristic of the LTR retrotransposons. Additionally, both LTR and non-LTR retrotransposons use distinct mechanisms of mobilization. Non-LTR retrotransposons are further divided into two classes - Long interspersed nuclear elements (LINEs) and short interspersed nuclear elements (SINEs), both of which occur abundantly in most mammals, including humans. Some of the active non-LTR retrotransposons in humans are L1...