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Updated: Jun 29, 2025

Method for Measuring the Activity of Deubiquitinating Enzymes in Cell Lines and Tissue Samples
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Method for Measuring the Activity of Deubiquitinating Enzymes in Cell Lines and Tissue Samples

Published on: May 10, 2015

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一个细胞透的Ub-Dha探针用于在活细胞中分析E1-E2-E3酶.

Qiong Xia1, Xianbin Meng2, Yu Wang1

  • 1School of Food and Biological Engineering, Engineering Research Center of Bio-process, Ministry of Education, Hefei University of Technology, Hefei 230009, China. ymli@hfut.edu.cn.

Chemical communications (Cambridge, England)
|March 28, 2024
PubMed
概括

研究人员开发了新的细胞透性无素探针 (Ub-ABP) 来研究活细胞中的E1-E2-E3酶活性. 这些探针使得在完整的细胞内追踪乌比基酶功能成为可能,克服了细胞溶解酸盐研究的先前局限性.

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Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
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