UBE2S的损失导致半体分裂I停止与正常的组装检查点动态在小鼠卵细胞
Si-Min Sun1,2,3,4, Bing-Wang Zhao1,2,3,4, Yuan-Yuan Li1,2,3
1State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China.
Development (Cambridge, England)
|March 28, 2024
概括
E2无素结合酶UBE2S对于小鼠卵细胞的成熟和生育是至关重要的. 缺少它会导致半变异I停止,影响APC/C活动,导致早期胚胎死亡.
科学领域:
- 细胞生物学 细胞生物学
- 发展生物学 发展生物学
- 生殖生物学 生殖生物学
背景情况:
- 通过全方位蛋白-蛋白酶体系统降解蛋白质,对于细胞循环调节至关重要.
- 亚纳酶促进复合体/循环体 (APC/C) 是一个关键的E3泛基因结合酶,控制半变异.
- 乌比奎结合酶E2S (UBE2S) 参与了将乌比奎输送到APC/C,但其在卵细胞中的作用尚不清楚.
研究的目的:
- 调查UBE2S在小鼠卵细胞成熟和女性生育能力中的功能.
- 阐明UBE2S影响介质进展的分子机制.
主要方法:
- 对Ube2s淘汰赛小鼠的生成和分析.
- 显微镜检查卵细胞螺旋组合和检查点动态.
- 评估APC/C活动和基质水平 (B1环,securin).
- 受精和早期胚胎发育研究.
主要成果:
- 被Ube2s删除的卵细胞表现出半体变异I停止与完整的螺旋组件.
- 缺少UBE2S会影响APC/C活性,导致高水平的环素B1和securin.
- 在Ube2s缺乏的卵细胞中,同质染色体分离失败了.
- 受精卵细胞发展到植入,但在胚胎日前被捕并死亡 10.5.5.
结论:
- UBE2S在调节小鼠卵细胞化中发挥着不可或缺的作用.
- UBE2S对于适当的APC/C功能,同质染色体分离和女性生育能力至关重要.
- 破坏UBE2S功能会导致早期胚胎死亡.
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