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盐和热应激增强了蓝藻细菌中的产生

Panayiotis-Ilias Broussos¹, George E Romanos², Kostas Stamatakis³

¹Institute of Biosciences and Applications, NCSR Demokritos, Patr. Gregoriou E & Neapoleos 27, 15341 Agia Paraskevi, Attikis, Greece.

Photosynthesis research

|March 28, 2024

概括

蓝色细菌,比如Synechococcus elongatus PCC7942 PAMCOD和Synechocystis sp. 这样的细菌. PCC6714,可以设计用于二氧化碳捕获和生物燃料生产. 这项研究表明,它们在压力下增加了糖和的产量,证明了可持续的潜力.

科学领域:

生物技术是生物技术.
微生物学微生物学
环境科学环境科学

背景情况:

菌对碳捕获非常有效,可以在极端条件下壮成长.
这两种菌种是Synechococcus elongatus PCC7942 PAMCOD和Synechocystis sp. 这两种菌种. PCC6714 在盐度压力下合成糖糖作为兼容的溶解物.
以前的研究表明,PAMCOD菌株通过暗发酵产生气.

研究的目的:

在Synechococcus elongatus PCC7942 PAMCOD和Synechocystis sp.中研究糖积累和细胞增殖. 在盐和热应激下PCC6714.
为了证明和优化Synechocystis sp.中的生产. PCC6714通过暗无氧发酵.
评估这些蓝菌株在生物燃料生产中的可持续性.

主要方法:

暴露的蓝藻细菌菌株 (Synechococcus elongatus PCC7942 PAMCOD 和 Synechocystis sp. 这两种细菌). PCC6714) 盐 (0.4 M NaCl) 和热 (35°C) 的压力.
测量细胞内糖含量 (mol糖/Chl a) 和细胞增殖率.
为 (H2) 生产优化暗无氧发酵条件,并评估发酵后的细胞生长.

主要成果:

细胞内糖糖在PAMCOD中增加了50%,在Synechocystis sp.中增加了108%. 在压力条件下的PCC6714.
综合囊 sp. 的情况. 证实PCC6714通过暗发酵产生,类似于PAMCOD.
优化的发酵条件提高了PAMCOD的H2生产率,达到23nmol H2 h−1 mol Chl a−1.1.
在发酵后,这两种菌株都在BG-11介质中繁殖,这表明该过程的可持续性.

结论:

这两种菌种是Synechococcus elongatus PCC7942 PAMCOD和Synechocystis sp. 这两种菌种. 在盐度和热应激相结合的情况下,PCC6714表现出显著的糖积累.
综合囊 sp. 的情况. PCC6714是通过暗发酵生产的可行候选者.
这些蓝菌株显示出可持续生物燃料和碳水化合物生产的潜力,以及二氧化碳捕获能力.

关键词:

这种菌株是Synechococcus elongatus PCC7942 PAMCOD菌株.综合囊 sp. 的情况. PCC6714 生物化物蓝藻细菌是一种蓝藻细菌.黑暗发酵黑暗发酵

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