优化从乳牛呼出的气息中取样挥发性有机化合物,使用聚合物基固相提取盒进行气体染色学分析
Julia Eichinger1,2, Anna-Maria Reiche1, Frigga Dohme-Meier1
1Ruminant Nutrition and Emissions, Agroscope, Posieux, Switzerland.
选择合适的固体相提取 (SPE) 弹对牛气中的挥发性有机化合物 (VOC) 的检测有重大影响. 不同的SPE弹捕获不同的数量和类型的VOC,影响气色谱/质谱 (GC-MS) 分析.
科学领域:
- 环境科学 环境科学
- 分析化学 分析化学
- 动物科学动物科学
背景情况:
- 呼出的呼吸中的挥发性有机化合物 (VOC) 提供了关于动物健康和新陈代谢的见解.
- 准确检测挥发性有机物需要优化技术设置,特别是在样品制备中.
- 固相提取 (SPE) 是集中和分析VOC的关键步骤.
研究的目的:
- 评估和比较六种商用聚合物基SPE弹的性能,以检测牛呼出的呼吸中的VOCs.
- 通过气体染色学/质谱学 (GC-MS) 确定不同的SPE弹如何影响检测到的VOC的种类和数量.
- 为在牛呼吸道样本中进行有针对性和非有针对性的VOC分析提供关于选择合适的SPE弹药盒的指导.
主要方法:
- 从五只正在哺乳的奶牛那里收集出口气样本.
- 六种不同的SPE弹 (Bond Elut ENV,Cromabond HRX,HRP,HLB,HR-XCW和HR-XAW) 用于样品捕获.
- 捕获的VOC被分析使用动态头空间真空管内提取与GC-MS (DHS-V-ITEX-GC-MS) 结合.
主要成果:
- 每个弹检测到1174至1312个VOC,其特异性和检测到的化合物的数量在弹之间有显著差异.
- XAW弹对子具有最高的特异性,并捕获了最多的特定VOCs (149).
- 总共有176种不同的VOC被确定,匹配系数>80%,突出显示了SPE弹选择对分析结果的重大影响.
结论:
- 所有经过测试的SPE弹都适合从奶牛口气中采集VOC样本,但弹的选择极大地影响了检测到的化学特征.
- 选择SPE弹应以特定的挥发性有机物或有趣的化学组为指导目标代谢学.
- 对于非有针对性的方法,建议使用多吸收剂弹或每个动物的多个弹来最大限度地检测VOC.
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