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Updated: Jul 18, 2026

Efficient Generation of hiPSC Neural Lineage Specific Knockin Reporters Using the CRISPR/Cas9 and Cas9 Double Nickase System
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使用CRISPR/Cas9系统 (WAe001-A-2C) 生成SST-P2A-mCherry记者人类胚胎干细胞系

Tongran Zhang1, Feng Zhang2, Nannan Wang3

  • 1Department of Pathophysiology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430074, China; Guangzhou Laboratory, Guangzhou 510006, China.

Stem cell research
|March 28, 2024
PubMed
概括

研究人员创建了一个人类胚胎干细胞系,该干细胞系报告了体静止素的产生. 这种新工具有助于研究胰腺三角细胞和开发糖尿病治疗方法.

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相关概念视频

CRISPR01:59

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...