相关实验视频

Updated: Jun 29, 2025

The Application of Open Searching-based Approaches for the Identification of Acinetobacter baumannii O-linked Glycopeptides

The Application of Open Searching-based Approaches for the Identification of Acinetobacter baumannii O-linked Glycopeptides

Published on: November 2, 2021

聚亚尼林涂层静止相在液友相互作用中的潜力基于液色谱的固态相提取用于糖丰富的聚亚尼林涂层静止相

Katarina Molnarova¹, Tomas Krizek¹, Petr Kozlik¹

¹Department of Analytical Chemistry, Faculty of Science, Charles University, Prague, Czech Republic.

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences

|March 28, 2024

概括

这项研究表明,聚亚尼林涂层的凝作为一种有效的材料,用于通过水友互动液体染色学 (HILIC) 分析人类免疫球蛋白G (IgG) 的糖丰富. 优化化和洗条件显著提高了糖丰富的效率和选择性.

科学领域:

分析化学分析化学
生物化学生化学
材料科学材料科学材料科学

背景情况:

糖丰富对于糖蛋白组学分析至关重要.
在极性静止相上通常使用水友互动液态色谱 (HILIC) 与固相提取 (SPE).
需要新的吸附材料来提高缩效率和选择性.

研究的目的:

评估聚氨酸 (PANI) 涂层的凝作为一种用于糖丰富的新型SPE吸收剂.
为了研究实验条件对使用PANI涂层凝的糖丰富效率的影响.
为了比较PANI涂层棉花SPE与常规棉花SPE,对人类免疫球蛋白G (IgG) 糖进行分析.

主要方法:

用聚氨 (PANI) 涂层的凝作为SPE吸附剂的制备.
固体相提取 (SPE) 与水友互动液体色谱 (HILIC) 结合用于糖丰富.
实验参数的系统变化:酸度,酸替代,酸度在化溶剂中,酸度在调节/洗溶液中.

主要成果:

化溶剂中的高甲基酸度促进了糖的早期化,特别是化的.
酸在化溶剂中增强了中性糖与PANI吸附剂的相互作用.
在调节和洗溶液中优化乙二的度对于糖的保留和丰富效率至关重要,较低的度导致最少的保留.

结论:

内部制备的PANI修饰吸附剂显示出SPE-HILIC糖丰富的显著潜力.
仔细调整实验条件,特别是溶剂成分,对于最大限度地提高缩效率和选择性至关重要.
这种方法为增强复杂的生物样本 (如人类IgG) 的糖蛋白组分析提供了一个有希望的策略.

关键词:

富含葡萄糖的丰富剂螺旋式的螺旋式的免疫球蛋白G是一种免疫球蛋白.聚氨氨基烯的使用方法固体相提取方法固体相提取方法

更多相关视频

Glycomics-Guided Glycoproteomics Facilitates Comprehensive Profiling of the Glycoproteome in Complex Tumor Microenvironments

Glycomics-Guided Glycoproteomics Facilitates Comprehensive Profiling of the Glycoproteome in Complex Tumor Microenvironments

Published on: February 7, 2025

A Spin-Tip Enrichment Strategy for Simultaneous Analysis of N-Glycopeptides and Phosphopeptides from Human Pancreatic Tissues

A Spin-Tip Enrichment Strategy for Simultaneous Analysis of N-Glycopeptides and Phosphopeptides from Human Pancreatic Tissues

Published on: May 4, 2022

相关概念视频

High-Performance Liquid Chromatography: Introduction

High-Performance Liquid Chromatography: Introduction

High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
In HPLC, two phases play a critical role in the separation process:

Gas Chromatography: Types of Columns and Stationary Phases

Gas Chromatography: Types of Columns and Stationary Phases

Gas chromatography (GC) relies on stationary phases to separate and analyze components in a sample. There are two main types of stationary phases: liquid and solid. Liquid stationary phases are non-volatile, thermally stable, and chemically inert liquids coated onto the column. Solid stationary phases are particles of adsorbent material, such as silica gel or molecular sieves.
For an analyte to remain on the column for a sufficient amount of time, it must exhibit some level of compatibility (or...

Size-Exclusion Chromatography

Size-Exclusion Chromatography

In size-exclusion chromatography (SEC), also known as molecular-exclusion or gel-permeation chromatography, molecules are separated based on their sizes. This technique is important for separating large molecules such as polymers and biomolecules. The two classes of micron-sized stationary phases encountered in SEC are silica particles and cross-linked polymer resin beads. Both materials are porous, but their pore sizes vary significantly.
Silica particles offer advantages such as rigidity,...

High-Performance Liquid Chromatography: Elution Process

High-Performance Liquid Chromatography: Elution Process

In High-Performance Liquid Chromatography (HPLC), the elution process is critical to the separation of analytes and the quality of chromatographic results. Elution describes how compounds move through the column and separate based on their interactions with the mobile and stationary phases. This process determines the resolution, peak shape, and retention times in the chromatogram, which are essential for identifying and quantifying components in complex mixtures. Understanding the elution...

Analyte Adsorption and Distribution

Analyte Adsorption and Distribution

In certain chromatographic separations, solutes transfer between the mobile phase and the stationary phase via sorption, which typically refers to the process of adsorption. For many chromatographic systems, the sorption process often depends on the polarity of the compounds—an expression of the overall dipole moment within the molecule. During the separation process, there is competition between the solute and solvent for adsorption to the stationary phase. Highly polar compounds and...

Affinity Chromatography

Affinity Chromatography

Affinity chromatography is a powerful technique extensively utilized for separating and purifying specific biomolecules from complex mixtures. It capitalizes on the highly selective binding between an analyte and its counterpart, such as antibody-antigen interactions. The counterpart is immobilized on the stationary phase, forming an affinity column. The stationary phase typically consists of solid support, such as agarose or porous glass beads, immobilizing the affinity ligand. The mobile...