相关实验视频

Updated: Jun 29, 2025

Lung microRNA Profiling Across the Estrous Cycle in Ozone-exposed Mice

Lung microRNA Profiling Across the Estrous Cycle in Ozone-exposed Mice

Published on: January 7, 2019

在高温压力或振动刺激后,太平洋中的外体miRNAs的表达模式

Lu Liu¹, Lei Gao¹, Keli Zhou¹

¹Liaoning Key Laboratory of Marine Animal Immunology and Disease Control, Dalian Ocean University, Dalian, 116023, China; Dalian Key Laboratory of Aquatic Animal Disease Prevention and Control, Dalian Ocean University, Dalian, 116023, China.

Developmental and comparative immunology

|March 28, 2024

概括

太平洋鱼的海.

科学领域:

海洋生物学海洋生物学
分子生物学分子生物学
基因组学就是基因组学.

背景情况:

外体微RNAs (miRNAs) 对细胞间通信至关重要,特别是在对环境压力和病原体的反应中.
太平洋 (Crassostrea gigas) 是一个经济重要物种,容易受到环境变化和疾病的影响.

研究的目的:

在高温压力和Vibrio splendidus刺激下研究Crassostrea gigas中外体miRNA的表达特征.
识别差异表达的miRNA及其目标基因,阐明它们在应激反应中的作用.

主要方法:

使用高通量测序来分析外体miRNA表达.
传输电子显微镜的特征是外体体形态.
定量PCR (qPCR) 验证了miRNA的表达.
基因本体学 (GO) 和基因和基因组的京都百科全书 (KEGG) 分析预测了目标基因功能.

主要成果:

确定了66种已知的和33种新型miRNA;在两种压力条件下,有10种被差异地表达.
氨酸阿斯巴达酶1-样是一种突出的标基因.
高温压力丰富了与亡相关的基因.
振动体刺激丰富了与自和细胞因子活动相关的基因.
能量代谢是两种压力因素的共同丰富途径.

结论:

外体miRNAs在调解Crassostrea gigas对环境压力和病原体挑战的反应中发挥着重要作用.
特定的miRNA点与不同的压力反应有关,包括亡,自和免疫信号.
了解这些监管机制对于养殖的弹性至关重要.

关键词:

这种植物是Crassostrea gigas.能量代谢能量代谢外体的miRNAs可以使用.高温应力压力是什么高温应力压力振动刺激是一种振动刺激.

更多相关视频

Temporal Quantification of MAPK Induced Expression in Single Yeast Cells

Temporal Quantification of MAPK Induced Expression in Single Yeast Cells

Published on: October 4, 2013

Purification and microRNA Profiling of Exosomes Derived from Blood and Culture Media

Purification and microRNA Profiling of Exosomes Derived from Blood and Culture Media

Published on: June 14, 2013

相关概念视频

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...

Nuclear Export of mRNA

Nuclear Export of mRNA

Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...