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在初级活体样本中的生物对等光催化接近性标记

Ziqi Liu^1,2, Fuhu Guo¹, Yufan Zhu¹

¹Synthetic and Functional Biomolecules Center, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, Beijing National Laboratory for Molecular Sciences, College of Chemistry and Molecular Engineering, Peking University, Beijing, China.

Nature communications

|March 29, 2024

概括

一种名为CAT-S的新方法,可以在现场在活细胞和组织中进行线粒体蛋白质组分析. 这项技术揭示了隐藏的蛋白质和与疾病相关的代谢变化,进步了我们对细胞过程和疾病的理解.

科学领域:

生物化学生物化学
细胞生物学细胞生物学
蛋白质组学是指蛋白质组学.

背景情况:

在原始生物系统中的in situ亚细胞蛋白质组对于理解生命过程和疾病至关重要.
方法上的挑战阻碍了对原生组织和临床样本进行全面的蛋白质组分析.

研究的目的:

开发一种用于在不同初级生物样本中的线粒体蛋白质体的现场分析的新方法.
扩大近距离标签的实用性,用于调查原生状态蛋白质特征和与疾病相关的变化.

主要方法:

CAT-S (化学启用近距离标记) 使用生物对称的光催化方法,使用 thioQM 标记弹头.
该方法使 mitochondrial 蛋白质能够在活细胞,组织和初级 T 细胞中进行高效和特定的标记.
适用于各种样本,包括细胞培养,分离的小鼠组织和人类血液T细胞.

主要成果:

在各种初级样本中,CAT-S成功地分析了原生状态的线粒体蛋白质组.
鉴定了以前隐藏的线粒体蛋白质:PTPN1,SLC35A4 uORF,以及TRABD.
糖尿病小鼠中的量化蛋白质组扰动,揭示与疾病进展相关的脂质代谢变化.

结论:

在亚细胞蛋白质组调查中,CAT-S提供了一个强大的非遗传工具,用于时空分辨率.
该方法广泛适用于初级样本,克服了以前的可访问性限制.
CAT-S为研究本地生物系统中的细胞机制和疾病病理开辟了新的途径.

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