多参数液体活检方法可以追踪黑色素瘤的动态,并确定早期的治疗耐药性
Maria Chiara Scaini1, Cristina Catoni2, Cristina Poggiana3
1Immunology and Molecular Oncology Unit, Veneto Institute of Oncology - IOV IRCCS, Padua, Italy. mariachiara.scaini@iov.veneto.it.
NPJ precision oncology
|March 29, 2024
概括
液体活检,使用循环瘤DNA (ctDNA) 和循环黑色素瘤细胞 (CMC),有效地跟踪黑色素瘤的演变和治疗反应. 这种方法有助于监测转移性黑色素瘤患者的疾病进展和耐药性.
科学领域:
- 在瘤学瘤学.
- 遗传学 遗传学 是一个
- 生物标志物 生物标志物
背景情况:
- 黑色素瘤的异质性使转移性疾病的管理变得复杂.
- 向治疗改善了结果,但耐药性需要持续监测.
- 液体活检提供了实时的疾病进化追踪.
研究的目的:
- 为了将液体活检动态与治疗反应和黑色素瘤的进展相关联.
- 为纵向患者监测建立一个多平台的方法.
- 研究循环瘤DNA (ctDNA) 和循环黑色素瘤细胞 (CMC) 的动态.
主要方法:
- 应用了使用下一代测序 (NGS),滴滴数字PCR和CellSearch的多平台方法.
- 分析了ctDNA趋势,CMC计数和遗传/复制数变异.
- 监测了17名IV期黑色素瘤患者,他们接受了BRAF/MEK抑制剂治疗长达28个月.
主要成果:
- 在82%的患者的血中检测到BRAF突变.
- 在70%的患者中发现了与耐药性相关的变异.
- ctDNA水平与治疗反应类型和持续时间相关;CMC数量具有预后性.
结论:
- 这种多平台液体活检方法有效地捕捉了黑色素瘤瘤的演变.
- ctDNA和CMC分析可以补充NGS用于疾病监测.
- 这些发现支持在第四阶段黑色素瘤中进一步验证早期ctDNA引导的治疗决策.
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