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Updated: Jul 26, 2026

Detection of Antibodies That Neutralize the Cellular Uptake of Enzyme Replacement Therapies with a Cell-based Assay
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开发和验证一种基于细胞的结合性中和抗体检测方法,用于抗体与药物结合物的检测

Weifeng Xu1, Nazneen Bano2, Olguitza Guzman-Valdes3

  • 1Merck & Co., Inc., Rahway, New Jersey, 07065, USA. Weifeng.xu2@merck.com.

The AAPS journal
|March 29, 2024
PubMed
概括

开发抗体-药物合物 (ADC) 的中和抗体 (NAb) 测定对癌症治疗至关重要. 当基于细胞的杀死试验由于意外结果而失败时,开发了一种新的基于细胞的结合试验.

关键词:
Fc受体 (FcγRs) 是一种中微尺度发现 (MSD)抗体药物合物 (ADC) 是一种抗体.基于细胞的杀死试验.免疫原性测试 免疫原性测试中和抗体 (NAb) 的使用.

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相关概念视频

Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.