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相关概念视频

Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

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Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
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相关实验视频

Updated: Jun 29, 2025

Quantification of Site-specific Protein Lysine Acetylation and Succinylation Stoichiometry Using Data-independent Acquisition Mass Spectrometry
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数据独立获取 代组学

Leon Bichmann1, Shubham Gupta2, Hannes Röst2

  • 1Department of Computer Science, Applied Bioinformatics, University of Tübingen, Tübingen, Germany.

Methods in molecular biology (Clifton, N.J.)
|March 29, 2024
PubMed
概括
此摘要是机器生成的。

在质谱学中,数据独立采集 (DIA) 提供了更好的可重现性和灵敏性. 迪亚蛋白质组学管道自动化了蛋白质组学和基组学数据的高通量分析,使得更多的研究人员可以访问迪亚蛋白质组学.

关键词:
自动化数据分析数据分析.生物质谱仪生物质谱仪时间 时间 时间 时间下一个流程 下一个流程类组学 类组学 类组学蛋白质组学是指蛋白质组学.斯瓦特 (SWATH) 是一个流浪汉.

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Proteomic Profile of EPS-Urine through FASP Digestion and Data-Independent Analysis
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科学领域:

  • 生物质谱仪生物质谱仪
  • 蛋白质组学是指蛋白质组学.
  • 类组学 类组学 类组学

背景情况:

  • 数据独立采集 (DIA) 是一种强大的质谱技术,比数据依赖采集 (DDA) 提供了增强的可重现性,灵敏度和动态范围.
  • 实施DIA用于高通量生物分析需要强大的计算管道来管理复杂的数据集.

研究的目的:

  • 介绍DIAproteomics,一个自动化,高通量计算管道用于处理蛋白质组学和组学DIA数据集.
  • 通过用户友好的工作流程,为非专家用户提高DIA质谱分析的可访问性.

主要方法:

  • 开发了DIAproteomics作为一个基于Nextflow的计算工作流.
  • 集成的OpenSwath工作流程用于染色体提取,峰值选择,评分,对齐和统计评估.
  • 使用光谱库 (现有或DDA生成) 进行DIA数据分析.

主要成果:

  • DIAproteomics 能够在各种计算基础设施中实现 DIA 数据集的高吞吐量处理.
  • 该管道通过命令行使用方便对类药物数据进行定性和定量分析.
  • 工作流是开源的,可在允许许可证下进行社区使用和修改.

结论:

  • 迪亚蛋白质组学显著降低了在生物研究中使用迪亚质谱的进入壁垒.
  • 该管道支持大规模蛋白质组学和组学研究的可复制和敏感分析.
  • 鼓励社区采用和适应DIAproteomics以满足特定的研究需求.