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Luyao Xue¹, Yuxin Wang¹, Yue Fan¹

¹Key Laboratory of Sweet Potato Biology and Biotechnology, Ministry of Agriculture and Rural Affairs/Beijing Key Laboratory of Crop Genetic Improvement/Laboratory of Crop Heterosis & Utilization and Joint Laboratory for International Cooperation in Crop Molecular Breeding, Ministry of Education, College of Agronomy & Biotechnology, China Agricultural University, Beijing, 100193, China.

The Plant journal : for cell and molecular biology

|March 29, 2024

概括

降低甜 (Ipomoea batatas) 中的IbNF-YA1基因的调节可以通过调节英多尔-3-酸 (IAA) 生物合成来提高储存根的产量,这为改善作物提供了新的策略.

科学领域:

植物生物学植物生物学
农业科学农业科学
分子遗传学分子遗传学

背景情况:

甜 (Ipomoea batatas) 储存的根产对全球粮食安全至关重要.
了解控制储存根形成的遗传机制是一个关键的研究领域.
以前的转录组分析确定了参与储存根发育的候选基因.

研究的目的:

为了研究IbNF-YA1基因在甜储存根产中的作用.
阐明IBNF-YA1,auxin (英多-3-酸 - IAA) 和储存根形成之间的关系.
探索操纵IbNF-YA1以提高甜产量的潜力.

主要方法:

使用转录组数据和qRT-PCR进行基因表达分析.
转基因甜植物的生成具有IBNF-YA1过度表达 (OE) 和RNA干扰 (RNAi).
在转基因和野生类型植物中测量储存的根产量和内源性IAA含量.
分析IBNF-YA1和IAA生物合成基因 (IbYUCCA家族) 之间的调控关系.

主要成果:

IbNF-YA1基因表达是由IAA诱导的.
过度表达IBNF-YA1降低了储存根的产量,而RNAi增加了它.
IbNF-YA1 OE植物显示IAA含量增加;RNAi植物显示IAA含量减少.
IbNF-YA1直接准并激活IBYUCCA4,这是IAA生物合成中的关键基因.
过度表达IbYUCCA4显著降低了储存根的产量.

结论:

IbNF-YA1基因在甜储存的根产中起着负面的调节作用.
通过调节IAA生物合成,特别是通过IbYUCCA4.4,IbNF-YA1影响储存根的产量.
降低IBNF-YA1的调节是一种有希望的策略,可以提高甜的储存根产量.

关键词:

在IbNF-YA1中.这就是IBYUCCA4的原因.辅助剂辅助剂辅助剂根存储根存储根存储甜是一种甜.收益率收益率收益率收益率

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