Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Computational Structural Analysis Predicts Host-Range Promiscuity and Antiviral Resistance in North American H5N1 Lineages.

Computational and structural biotechnology journal·2026
Same author

Meet Our Editorial Board-Genesis. An Interview With Yevgenya Grinblat, University of Wisconsin-Madison, Wisconsin, USA.

Genesis (New York, N.Y. : 2000)·2025
Same author

Using Apple Watches to Monitor Health and Behaviors of Individuals With Cognitive Impairment: A Case Series Study.

The journals of gerontology. Series A, Biological sciences and medical sciences·2024
Same author

Cadherin 16 promotes sensory gating via the endocrine corpuscles of Stannius.

bioRxiv : the preprint server for biology·2024
Same author

PD-1 Targeted Antibody Discovery Using AI Protein Diffusion.

Technology in cancer research & treatment·2024
Same author

Zebrafish models of alx-linked frontonasal dysplasia reveal a role for Alx1 and Alx3 in the anterior segment and vasculature of the developing eye.

Biology open·2022

相关实验视频

Updated: Jun 29, 2025

Behavioral And Physiological Analysis In A Zebrafish Model Of Epilepsy
00:08

Behavioral And Physiological Analysis In A Zebrafish Model Of Epilepsy

Published on: October 19, 2021

5.6K

在斑马鱼中对mecp2功能的行为和转录组分析.

Nicholas J Santistevan1,2, Colby T Ford3,4,5, Cole S Gilsdorf1

  • 1Departments of Integrative Biology and Neuroscience, University of Wisconsin, Madison, Wisconsin, USA.

American journal of medical genetics. Part B, Neuropsychiatric genetics : the official publication of the International Society of Psychiatric Genetics
|March 29, 2024
PubMed
概括

斑马鱼的mecp2突变显示出改变的tighmotaxis和视觉反应,揭示了甲基-CpG结合蛋白2 (MECP2) 在脊椎动物神经发育中的保留作用. 这项研究强调了斑马鱼作为理解雷特综合征MECP2功能的补充模型.

关键词:
这就是MeCP2的意义.雷特综合征 (RTT) 是一个行为分析行为分析.转录组分析 转录组分析斑马鱼是一种斑马鱼.

更多相关视频

Zebrafish In Situ Spinal Cord Preparation for Electrophysiological Recordings from Spinal Sensory and Motor Neurons
08:24

Zebrafish In Situ Spinal Cord Preparation for Electrophysiological Recordings from Spinal Sensory and Motor Neurons

Published on: April 18, 2017

11.4K
High Resolution Whole Mount In Situ Hybridization within Zebrafish Embryos to Study Gene Expression and Function
10:06

High Resolution Whole Mount In Situ Hybridization within Zebrafish Embryos to Study Gene Expression and Function

Published on: October 19, 2013

22.2K

相关实验视频

Last Updated: Jun 29, 2025

Behavioral And Physiological Analysis In A Zebrafish Model Of Epilepsy
00:08

Behavioral And Physiological Analysis In A Zebrafish Model Of Epilepsy

Published on: October 19, 2021

5.6K
Zebrafish In Situ Spinal Cord Preparation for Electrophysiological Recordings from Spinal Sensory and Motor Neurons
08:24

Zebrafish In Situ Spinal Cord Preparation for Electrophysiological Recordings from Spinal Sensory and Motor Neurons

Published on: April 18, 2017

11.4K
High Resolution Whole Mount In Situ Hybridization within Zebrafish Embryos to Study Gene Expression and Function
10:06

High Resolution Whole Mount In Situ Hybridization within Zebrafish Embryos to Study Gene Expression and Function

Published on: October 19, 2013

22.2K

科学领域:

  • 神经科学是一个神经科学.
  • 遗传学 是一个遗传学.
  • 发展生物学 发展生物学

背景情况:

  • 雷特综合征 (RTT) 是一种由甲基-CpG结合蛋白2 (MECP2) 功能障碍引起的神经发育障碍.
  • 鼠标模型至关重要,但不能完全解释MECP2机制.
  • 斑马鱼的mecp2突变已经显示出轻微的缺陷,但需要更深入的研究.

研究的目的:

  • 为了全面评估斑马鱼mecp2突变的行为和转录组变化.
  • 研究mecp2在运动反应,感官过和视觉行为中的作用.
  • 在脊椎动物中识别由MeCP2调节的保存途径.

主要方法:

  • 在受精后4天进行转录基因分析 (RNA-seq).
  • 在斑马鱼突变体 (5-7 dpf) 中评估运动反应和感官处理的行为测试.
  • 斑马鱼和哺乳动物系统之间的比较途径分析.

主要成果:

  • 斑马鱼的mecp2对于正常的tighmotaxis和视觉刺激反应至关重要.
  • 对于总运动活动,声学惊或感官过 (习惯,感官运动门) 不需要Mecp2功能.
  • RNA-seq确定了许多需要mecp2进行正确转录的基因和途径,在脊椎动物中保留了功能.

结论:

  • 作为转录调节器的MeCP2的作用在斑马鱼和哺乳动物之间得到保护.
  • 斑马鱼 mecp2 突变体表现出特定的行为变化,包括视觉反应受损.
  • 斑马鱼作为对小鼠有价值的补充模型,用于研究雷特综合征和MECP2功能.