通过记录DNA中的分析物检测,使用复合酶记忆记录,进行长期的环境生物传感
Prashant Bharadwaj Kalvapalle1, Swetha Sridhar1, Jonathan J Silberg2,3,4
1Systems, Synthetic, and Physical Biology Graduate Program, Rice University, Houston, Texas, USA.
Applied and environmental microbiology
|March 29, 2024
概括
带有遗传记忆生物传感器的工程微生物可以记录8天的短暂化学暴露. 优化的设计提高了微生物行为现场环境监测的性能.
科学领域:
- 合成生物学 合成生物学
- 微生物工程是微生物的工程.
- 环境监测环境监测环境监测
背景情况:
- 传统的微生物生物传感器在复杂的环境中 (如土壤和废水) 面临着光学难以进入的挑战.
- 一个有前途的解决方案涉及生物传感器,这些生物传感器记录了长时间的短暂分析物暴露,以便以后检索.
- 基于重组酶的遗传记忆系统提供了一种稳定,长期记录微生物环境相互作用的策略.
研究的目的:
- 设计和测试复合酶记忆生物传感器,以稳定记录微生物中的分析物暴露.
- 克服最初设计的局限性,包括意外的DNA翻转和记忆丧失.
- 应用和验证这些改进的内存生物传感器在环境隔离物中用于现场应用.
主要方法:
- 开发复合酶记忆生物传感器来检测阿拉比诺斯和3-oxo-C12-L-homoserine乳.
- 通过调整复合酶表达和输出机制来代优化生物传感器设计.
- 使用定量PCR来读取存储的遗传记忆.
- 在8天的时间内测试废水微生物分离物中的生物传感器性能.
主要成果:
- 最初的生物传感器设计表现出不稳定性,有无意的DNA翻转和记忆丧失.
- 优化的设计,包括减少复合酶表达和定量PCR读数,证明了性能的提高.
- 记忆生物传感器成功地记录了从废水中分离的非特征化*Pseudomonas*中分析物的暴露.
- 该系统在大约70代人的时间里表现出稳定的记忆.
结论:
- 再组合酶记忆生物传感器可以设计为稳定,长期记录微生物分析剂暴露.
- 优化策略对于在复杂的环境条件下可靠的性能至关重要.
- 这些工程微生物为微生物信号,社区动态和生态过程的实地调查提供了强大的工具.
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