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工程构成均的酵母全细胞生物催化剂,具有最大的表面酶密度,用于生产纤维素生物燃料

Xiao Yin Ma^1,2, Bryan Coleman^1,2, Ponnandy Prabhu¹

¹Department of Chemical Engineering, University of Michigan, Ann Arbor, Michigan 48109, United States.

ACS synthetic biology

|March 29, 2024

概括

研究人员设计了酵母全细胞生物催化剂 (yWCB),以改善生物乙醇生产的纤维素水解. 它们在群体和单细胞水平上增强了酶的表现,实现了近乎理论的产量.

科学领域:

生物技术是生物技术.
合成生物学合成生物学
生物化学工程生物化学工程

背景情况:

全细胞生物催化对于食品,制药和能源行业至关重要.
显示微细胞体的乙醇酵母为纤维素乙醇提供了整合生物处理.
目前的酵母全细胞生物催化剂 (yWCB) 显示纤维素水解效率低,限制了工业可行性.

研究的目的:

为了最大限度地提高yWCBs上的酶密度,以增强纤维素水解.
为了解决表现为四功能迷你细胞体的yWCBs在群体和单细胞水平上的局限性.

主要方法:

识别并消除了等离子体损失,以创建统一的yWCB群体.
操纵集成基因拷贝数以控制单细胞酶显示并克服分子拥挤.
为增强的微细胞体组装和表面显示而设计的yWCB.

主要成果:

通过消除非显示细胞,生成组成均的yWCBs.
达到可调节的酶显示水平,避免拥挤限制并最大限度地提高每个细胞的酶密度.
优化菌株的产量为4.92g/g的纤维素-乙醇 (96%的理论最大值),纤维素转化率为96%左右.

结论:

结合人口和单细胞方法的整体工程策略显著提高了yWCB的性能.
这种方法广泛适用于改进其他全细胞生物催化级联方案.
在纤维素乙醇生产中获得了工业相关的产量.

关键词:

生物乙醇生物乙醇的使用纤维素纤维素的使用方法纤维素体细胞体是什么?在构成上均,均.酶密度酶密度酶密度分子拥挤是分子拥挤.全细胞生物催化剂全细胞生物催化剂酵母表面显示器显示酵母表面.

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