一种依赖的三糖转运体,在昆虫细胞系Pv11中,有助于无水生菌
Kosuke Mizutani1, Yuki Yoshida2, Eita Nakanishi1
1Department of Integrated Biosciences, Graduate School of Frontier Science, The University of Tokyo, Kashiwa, Chiba 277-8562, Japan.
概括
研究人员发现了STRT1,一种新型的载体,对动物细胞在无水生菌病 (干燥) 后的恢复至关重要. 这种离子三醇转运器1促进了三醇的释放,改善了细胞活力和在再水后的形态恢复.
科学领域:
- 细胞生物学 细胞生物学
- 生物化学 生物化学
- 生物物理学的生物物理.
背景情况:
- Pv11细胞系表现出显著的无水生菌,在室温下在干燥状态下存活超过一年.
- 无水生菌症是一种极端的干燥耐受性,允许细胞在干燥后恢复增殖.
- 了解无水生菌病恢复的分子机制对于保存生物材料至关重要.
研究的目的:
- 确定参与Pv11细胞从无水生菌病中恢复的分子参与者.
- 描述一种新型的载体,该载体负责促进细胞存活和在再水化后的形态恢复.
- 阐明三糖运输在减轻再生过程中的压力中的作用.
主要方法:
- 使用异质表达系统来测试传送器活性.
- 在Pv11细胞中对已识别的载体 (Strt1) 进行基因淘汰.
- 细胞活力,三醇释放动力学和形态恢复在干燥和再水后被评估.
主要成果:
- 发现了一种新型的溶解物载体5 (SLC5) 家族成员STRT1 (离子三糖载体1).
- STRT1表现出Na+依赖的三运输活性,其表达在再水时增加.
- 绝杀Strt1显著降低了细胞活力,减缓了三糖的释放,并在再水化后延迟了形态恢复.
结论:
- STRT1在Pv11细胞从无水生菌病中恢复起着至关重要的作用.
- STRT1通过通过Na+依赖的三糖流动调节细胞内度来促进细胞生存.
- 这种载体对于缓解再水化过程中的身体压力至关重要,从而改善细胞活力并恢复正常形态.
相关概念视频
01:32Secondary Active Transport
7.0K
One example of how cells use the energy contained in electrochemical gradients is demonstrated by glucose transport into cells. The ion vital to this process is sodium (Na+), which is typically present in higher concentrations extracellularly than in the cytosol. Such a concentration difference is due, in part, to the action of an enzyme "pump" embedded in the cellular membrane that actively expels Na+ from a cell. Importantly, as this pump contributes to the high concentration of...
7.0K
01:47Osmoregulation in Insects
16.2K
Malpighian tubules are specialized structures found in the digestive systems of many arthropods, including most insects, that handle excretion and osmoregulation. The tubules are typically arranged in pairs and have a convoluted structure that increases their surface area.
16.2K
01:18Protein Transport to the Inner Chloroplast Membrane
2.1K
Proteins targeted to the inner chloroplast membrane, or plastid proteins, are transported by two general pathways: the stop-transfer and the re-insertion or post-import pathways. Most plastid proteins carry N-terminal transit sequences and internal import sequences targeting it to the specific chloroplast subcompartment. Proteins targeted by the stop-transfer pathway have internal hydrophobic sequences that inhibit their translocation into the stroma. As a result, these precursors are arrested...
2.1K
02:12Short-distance Transport of Resources
16.0K
Short-distance transport refers to transport that occurs over a distance of just 2-3 cells, crossing the plasma membrane in the process. Small uncharged molecules, such as oxygen, carbon dioxide, and water, can diffuse across the plasma membrane on their own. In contrast, ions and larger molecules require the assistance of transport proteins due to their charge or size. Transport across membranes also occurs within individual cells, playing a variety of essential roles for the plant as a whole.
16.0K
01:30Membrane Proteins
19.4K
Plasma membranes have integral transmembrane proteins involved in facilitated transport. These proteins are collectively referred to as transport proteins, and they function as either channels for the material or as carriers themselves. Channel proteins have hydrophilic domains exposed to the intracellular and extracellular fluids and a hydrophilic channel through their core that provides a hydrated opening for solutes to pass through the membrane layers. Passage through the channel allows...
19.4K
01:23Transcellular Transport of Solutes
3.6K
Transcellular transport of solutes is the movement of substances like monosaccharides and amino acids through polarized cells. This transport mechanism is primarily seen in epithelial and endothelial cells aided by membrane transport proteins such as channels and transporters. The tight junctions between these cells confine the membrane proteins to the two sides of the cell. The epithelial cells have distinct apical and basolateral domains. In contrast, the endothelial cells show the luminal...
3.6K