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来自病原体的高复制转体子产生了一个保存的sRNA家族,具有新的宿主免疫点

Lukas Kunz¹, Manuel Poretti^1,2, Coraline R Praz^1,3

¹Department of Plant and Microbial Biology, University of Zürich, Zollikerstrasse 107, CH-8008 Zürich, Switzerland.

Molecular plant-microbe interactions : MPMI

|March 29, 2024

概括

小麦粉病原体从转位子进化出植物微RNA (miRNA) 的功能模仿物. 这些模仿小RNAs (sRNAs) 准小麦基因,影响植物免疫力和宿主寄生虫进化.

科学领域:

进化生物学是进化的生物学.
植物病理学植物病理学
分子遗传学分子遗传学

背景情况:

小RNAs (sRNAs) 调解基因沉默,并且可以在物种之间转移.
进化的真核微生物对宿主调节RNA的功能模仿仍然不太了解.
短间隔核元素 (SINEs) 是移动的遗传元素,在宿主-病原体相互作用中起到很大程度上未知的作用.

研究的目的:

在小麦粉病原体中识别和描述新的小RNA (sRNA).
研究病原体衍生的sRNAs的进化起源和功能机制.
了解这些sRNA在宿主-病原体相互作用和植物免疫力中的作用.

主要方法:

病原体基因组的生物信息分析以确定sRNA家族.
使用分子生物学技术对已识别的sRNA进行功能性表征.
基因表达分析和植物免疫测定.

主要成果:

鉴定SINE_sRNA1,一种sRNA家族,其来源于小麦粉病原体SINE逆转移体.
SINE_sRNA1作为小麦微RNA (miRNA) 的功能模仿.
SINE_sRNA1的目标是Tae_AP1,一种增强模式触发免疫 (PTI) 和效应器触发免疫 (ETI) 的小麦基因.

结论:

转子子可以促进真核微生物中功能性小RNA模拟物的进化.
病原体衍生的sRNA可以操纵宿主免疫力,这表明它在宿主-寄生虫进化军备竞赛中的作用.
SINE_sRNA1和Tae_AP1代表了驱动植物防御机制的功能性创新.

关键词:

布卢梅里亚 (Blumeria) 是一种植物.亚斯帕尔特蛋白质酶是一种亚斯帕尔特蛋白质酶.跨王国的RNAi.可转移的元素可以转移.小麦小麦小麦小麦小麦小麦小麦.

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