超低载荷铜间接MoO3纳米带具有高活性对抗抗生素耐药细菌
Hu Liu1, Yuhui Zuo1, Shiyang Lv1
1College of Life Sciences, Institute of Biomedical Engineering, Qingdao University, Ningxia Road 308, Qingdao 266071, Shandong, China.
ACS applied materials & interfaces
|March 29, 2024
概括
铜合的MoO3纳米带作为纳米酶,通过产生活性氧物种和耗尽谷氨,有效杀死细菌. 这种新的方法为传统抗生素对抗多药耐药细菌提供了有希望的替代方案.
科学领域:
- 材料科学 材料科学 材料科学
- 纳米技术纳米技术
- 生物化学 生物化学
- 抗菌研究 抗菌研究
背景情况:
- 抗生素耐药性增加和多药耐药 (MDR) 细菌的流行构成了全球健康的重大威胁.
- 迫切需要新型抗生素替代品,具有广泛的抗菌活性.
- 层状材料通过介质提供可调节的特性,为开发新的功能纳米材料提供了一种战略.
研究的目的:
- 构建和评估铜合的α-MoO3纳米带作为抗菌应用中的纳米酶.
- 调查协同抗微生物机制,包括反应性氧物种 (ROS) 生成和谷氨 (GSH) 枯竭.
- 评估这些纳米带对临床相关的MDR细菌菌株的疗效.
主要方法:
- 合成与铜合的α-MoO3纳米带 (MoO3-/Cu).
- 材料性质和催化活性 (氧化酶,氧化酶,催化酶,谷氨氧化酶类) 的表征.
- 在细菌中对ROS生成和GSH耗尽的评估.
- 对ESBL-E. coli和MRSA的抗菌疗效的评估.
主要成果:
- 铜介质显著增强了α-MoO3纳米带的过氧化酶 (POD) 活性和GSH耗尽能力.
- MoO3-/Cu纳米带有效诱导POD,氧化酶 (OXD) 和催化酶 (CAT) 活动,从而产生大量的ROS.
- 这些纳米皮带显示出类似谷氨氧化酶 (GSHOx) 的活性,耗尽细胞内GSH并增强杀菌作用.
- 在低度 (20μg mL-1) 观察到对ESBL-E. coli和MRSA的优异抗菌效率.
结论:
- 铜合的MoO3纳米带作为具有强大的协同抗菌活性的多酶纳米酶起作用.
- 结合的ROS生成和GSH耗尽机制提供了对MDR细菌的有效策略.
- 这项研究强调了MoO3-/Cu纳米带的潜力,作为开发先进抗菌和抗感染剂的有希望的平台.
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