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Updated: Jun 29, 2025

Characterization of Neuronal Lysosome Interactome with Proximity Labeling Proteomics
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Characterization of Neuronal Lysosome Interactome with Proximity Labeling Proteomics

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在单蛋白分辨率下的神经元中的空间蛋白质组学

Eduard M Unterauer1, Sayedali Shetab Boushehri2, Kristina Jevdokimenko3

  • 1Max Planck Institute of Biochemistry, Planegg, Germany; Faculty of Physics and Center for NanoScience, Ludwig-Maximilians-Universität, Munich, Germany.

Cell
|March 29, 2024
PubMed
概括

我们开发了SUM-PAINT, 这是一种新的成像技术, 这种方法实现了高分辨率,揭示了细胞复杂性,并发现了神经元中的新突触类型.

关键词:
在DNA-PAINT激发性突触抑制性突触多重复制神经元图谱神经元成像蛋白质组学空间经济学超分辨率显微镜一个突触突触多样性突触蛋白质

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相关概念视频

Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
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Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
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