使用LC-MS/MS在血样本中的滥用药物的无溶剂并行人工液体膜提取
André Luis Fabris1, Stig Pedersen-Bjergaard2, Elisabeth Leere Øiestad3
1School of Pharmaceutical Sciences, University of São Paulo, São Paulo, SP, 05508-000, Brazil.
Analytica chimica acta
|March 29, 2024
概括
这项研究引入了一种更绿色的样本制备方法,用于药物分析,使用精油 (EO) 与并行人工液体膜提取 (PALME) 进行. 这种新技术有效地从血中提取滥用药物,取代危险的有机溶剂.
科学领域:
- 分析化学 分析化学
- 绿色化学 绿色化学
- 法医毒理学 法医毒理学
背景情况:
- 并行人工液体膜提取 (PALME) 是一种成熟的绿色提取技术.
- 这项研究旨在通过用精油 (EO) 取代有机溶剂来提高PALME的可持续性.
- 重点是分析血样本中的滥用药物,使用液体染色学-并联质谱法 (LC-MS/MS).
研究的目的:
- 开发和优化用于药物分析的无溶剂样本制备方法.
- 评估精油作为PALME中有机溶剂的替代品的有效性.
- 验证从血中提取各种滥用药物的方法.
主要方法:
- 十四种精油 (EO) 与常规有机溶剂进行分析回收的比较.
- 优化提取参数,包括时间,基度,样本体积和三氧化百分比,使用因子选和盒子-Behnken设计.
- 根据国际毒理学分析指南对优化方法的验证.
主要成果:
- 与有机溶剂相比,精油"smart & sassy"的分析物回收率更高.
- 优化的条件实现了高效的提取,具有特定的参数:120分钟的提取时间,10 mmol/L的NaOH,150μL的样本体积和0%的三氧化.
- 经过验证的方法表现出1-100 ng/mL的线性范围,准确度±16.4%,精度>83%,量化极限为1 ng/mL.
结论:
- 基于精油的PALME提供了一种无溶剂且高效的样本准备技术,用于药物滥用分析.
- 该方法通过消除危险的有机溶剂,与可持续化学原则保持一致.
- 开发的技术提供了优秀的样品清洁,适合常规毒理应用.
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