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Darren Brow¹, Hiroko Shike², Jasmine Kendrick¹

¹Department of Laboratory Medicine and Pathology, Mayo Clinic, Jacksonville, FL, USA.

Human immunology

|March 29, 2024

概括

下一代测序 (NGS) 提供了一种敏感且准确的方法,用于在异构造血型干细胞移植 (allo-HSCT) 后进行嵌合分析. 这种NGS测试简化了工作流程,并有效地监测了移植和最小残留疾病 (MRD).

科学领域:

分子生物学分子生物学
血液学血液学血液学
遗传学是一个遗传学.

背景情况:

化学主义分析对于对异构造血细胞干细胞移植 (allo-HSCT) 后的患者进行监测至关重要.
目前的方法结合短串重复 (STR) 和定量聚合酶连锁反应 (qPCR) 用于植入和最小可测量的残留疾病 (MRD) 监测.
需要更灵敏,更准确的嵌合式分析方法.

研究的目的:

评估基于插入/删除的多重合金测试,使用下一代测序 (NGS).
与传统的STR/qPCR方法相比,评估NGS试验的灵敏度,准确性和工作流程效率.
为了确定该测试在移植和MRD监测中对Allo-HSCT患者的有用性.

主要方法:

使用NGS开发并测试了一种基于插入/删除的多重合金测试.
模拟无关的供体-受体组合 (5),以及来自allo-HSCT受体 (50) 的外周血液样本进行了分析.
将NGS结果与传统的STR/qPCR方法进行比较,以确定相关性,偏差和一致性.

主要成果:

在NGS测试中,每个样本中检测到平均7-8个信息标记.
量化极限 (LoQ) 确定为0.1%的受体,总检测时间为19-31小时.
在检测受体增加≥0.1%时,NGS表现出强烈的相关性 (R2 = 0.9852) 和100%与STR/qPCR一致,表明高准确度和可靠性.

结论:

基于NGS的嵌合式测定是一种敏感,准确和可靠的方法,用于合金后HSCT监测.
这种NGS测定可以有效地支持所有allo-HSCT设置中的植入评估和MRD监测.
与传统的STR/qPCR方法相比,NGS试验提供了一个简化的临床实验室工作流程.

关键词:

异构造血型干细胞移植的异构造血型干细胞移植.化学主义测试测试化学主义测试可测量的残留疾病.下一代测序测序是什么复发复发的情况

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