雄激素受体-FL和V7转录的RNA二次结构揭示了新的调节区域
Warren B Rouse1, Van S Tompkins1, Collin A O'Leary1,2
1Roy J. Carver Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, IA 50011, USA.
Nucleic acids research
|March 30, 2024
概括
雄激素受体 (AR) 的mRNA结构影响其调节. 这项研究揭示了AR异型中的新型RNA结构,包括与PCBP2相互作用的5'UTR干循环,为AR表达控制提供了新的见解.
科学领域:
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
- 生物化学 生化学
背景情况:
- 雄激素受体 (AR) 对细胞生长和分化至关重要.
- AR失调与前列腺癌等疾病有关,通常在RNA水平.
- 对于AR mRNA的转录后调节,特别是RNA的二次结构,人们对其了解甚少.
研究的目的:
- 综合分析雄激素受体 (AR) mRNA的二次结构.
- 为了研究全长 (AR-FL) 和截断 (AR-V7) AR异型的结构景观和动态.
- 在AR mRNA中识别功能性重要的RNA结构,特别是在未翻译的区域.
主要方法:
- 使用细胞内RNA二次结构探测 (向的DMS-MaPseq).
- 采用计算预测来分析AR mRNA结构特征.
- 进行了细胞内测定,以评估AR-FL中确定的结构的功能相关性.
主要成果:
- 描述了AR-FL和AR-V7mRNA的静态结构和结构动态.
- 在AR-FL的5'和3'未翻译区域 (UTR) 中确定了功能相关的结构.
- 在AR-FL中发现了一种保存的5'UTR干循环,该循环结合了多RC结合蛋白2 (PCBP2).
结论:
- 新型RNA二次结构在调节AR表达方面发挥着重要作用.
- 已识别的AR mRNA结构和相互作用为了解AR失调提供了新的目标.
- 这项研究阐明了AR转录后控制的以前未知的机制.
相关概念视频
Cis-regulatory Sequences
9.9K
Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
9.9K
RNA Structure
71.4K
Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
71.4K
Transducer Mechanism: Nuclear Receptors
1.3K
Nuclear receptors, or NRs, are unique transcription factors that regulate gene transcription and affect the cellular pathways involved in reproduction, development, or metabolism. Their ability to be stimulated by small lipophilic ligands and control vital cellular processes makes them ideal drug targets. Nearly 10-15% of currently prescribed drugs target these receptors.
About 48 different soluble family members of nuclear receptors are identified that can be divided into two main classes:
About 48 different soluble family members of nuclear receptors are identified that can be divided into two main classes:
1.3K
Co-activators and Co-repressors
7.3K
Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
7.3K
Alternative RNA Splicing
21.1K
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
21.1K
Conserved Binding Sites
4.2K
Many proteins’ biological role depends on their interactions with their ligands, small molecules that bind to specific locations on the protein known as ligand-binding sites. Ligand-binding sites are often conserved among homologous proteins as these sites are critical for protein function.
Binding sites are often located in large pockets, and if their location on a protein’s surface is unknown, it can be predicted using various approaches. The energetic method computationally...
Binding sites are often located in large pockets, and if their location on a protein’s surface is unknown, it can be predicted using various approaches. The energetic method computationally...
4.2K


