相关实验视频
Updated: Jun 29, 2025

07:02
An Assay for Quantifying Protein-RNA Binding in Bacteria
Published on: June 12, 2019
6.6K
对于大型菌体感染,需要一种菌体核相关的RNA结合蛋白
Eray Enustun1, Emily G Armbruster1, Jina Lee1
1Department of Molecular Biology, University of California San Diego, La Jolla, CA 92093, USA.
Nucleic acids research
|March 30, 2024
概括
大型菌体使用类似于核的隔间. 一种叫做Chimallin C (ChmC) 的蛋白质对于将病毒mRNA移出这个容器至关重要,从而使蛋白质的产生和病毒组合成为可能.
科学领域:
- 病毒学 病毒学
- 分子生物学分子生物学
- 结构生物学 结构生物学
背景情况:
- 大型菌体 (大基因组菌体) 形成一个类似于核的对象,用于基因组保护.
- 这个区块需要对病毒复制和蛋白质合成的分子进行调节的运输.
研究的目的:
- 识别和表征涉及核细胞质运输在菌体核外中的蛋白质.
- 为了阐明一种新型菌体核相关蛋白的功能,Chimallin C (ChmC).
主要方法:
- 蛋白质净化和体外结合试验,以评估RNA相互作用.
- 在受感染细胞中识别ChmC及其与菌体mRNA的关联.
- 对ChmC的RNA结合和相分离研究.
- 通过CRISPR-Cas13d介导的ChmC的淘汰,以评估其体内功能.
主要成果:
- 鉴定了一种保存的菌体蛋白,Chimallin C (ChmC),表现出核酸结合特性和对RNA的高度亲和力.
- ChmC在感染细胞内结合了菌体mRNA,并形成了依赖RNA的分相凝聚物.
- Knockdown 的 ChmC 导致了 mRNA 在菌体核中的积累,减少了病毒蛋白质的产生,并损害了 virion 组装.
结论:
- 奇马林C (ChmC) 对于大型菌体生命周期至关重要.
- ChmC可能有助于病毒mRNA在核外的转移,这对于高效的蛋白质合成和病毒产生至关重要.
相关概念视频
Bacterial RNA Polymerase
29.5K
Unlike eukaryotes, bacteria use a single RNA Polymerase (RNAP) to transcribe all genes. The different subunits of bacterial RNAPhave distinct functions. The multisubunit structure of the bacterial RNAP helps the enzyme to maintain catalytic function, facilitate assembly, interact with DNA and RNA, and self-regulate its activity.
In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...
In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...
29.5K
RNA Polymerase II Accessory Proteins
9.2K
Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
9.2K
Lysogenic Cycle of Bacteriophages
62.1K
In contrast to the lytic cycle, phages infecting bacteria via the lysogenic cycle do not immediately kill their host cell. Instead, they combine their genome with the host genome, allowing the bacteria to replicate the phage DNA along with the bacterial genome. The incorporated copy of the phage genome is called the prophage. Some prophages can re-activate and enter the lytic cycle. This often occurs in response to a perturbation, such as DNA damage, but can also transpire in the absence of...
62.1K
Lytic Cycle of Bacteriophages
70.7K
Bacteriophages, also known as phages, are specialized viruses that infect bacteria. A key characteristic of phages is their distinctive “head-tail” morphology. A phage begins the infection process (i.e., lytic cycle) by attaching to the outside of a bacterial cell. Attachment is accomplished via proteins in the phage tail that bind to specific receptor proteins on the outer surface of the bacterium. The tail injects the phage’s DNA genome into the bacterial cytoplasm. In the...
70.7K
Regulation of Nuclear Protein Sorting
2.4K
Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...
2.4K
Retrovirus Life Cycles
45.9K
Retroviruses have a single-stranded RNA genome that undergoes a special form of replication. Once the retrovirus has entered the host cell, an enzyme called reverse transcriptase synthesizes double-stranded DNA from the retroviral RNA genome. This DNA copy of the genome is then integrated into the host’s genome inside the nucleus via an enzyme called integrase. Consequently, the retroviral genome is transcribed into RNA whenever the host’s genome is transcribed, allowing the...
45.9K

