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选择性基因表达和代谢物转化驱动在酸盐减少期间在交替的盛宴-饥荒条件下强大的酸盐积累

Duanyuan Xu¹, Rui Du¹, Shouyou Gao²

¹National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Engineering Research Center of Beijing, Beijing University of Technology, Beijing 100124, China.

|March 30, 2024

概括

一种新的宴会饥饿策略通过调节无化基因,有效地为anammox细菌积累酸盐. 这种方法增强了酸盐的生产,支持微生物社区的生存和废水处理应用.

科学领域:

环境微生物学环境微生物学
生物技术是生物技术.
废水处理废水处理

背景情况:

亚酸盐生产对于无氧氧化 (anammox) 细菌的生存至关重要.
基因表达和细胞外代谢物在亚酸盐积累中的作用仍未得到充分探索.

研究的目的:

开发和演示一个交替的聚餐-饥荒战略,以有效地积累酸盐.
研究碳源可用性对脱基因表达和微生物社区结构的影响.

主要方法:

在低加载率脱化系统中实施一种新型交替盛宴-饥荒策略.
对差异性基因表达的分析,重点关注narG,narI/V,nirS和nirK.
确定主导的脱细菌及其对关键基因的贡献.
监测细胞外代谢物 (氧化糖,酸盐,葡萄糖) 和它们对微生物群落的影响.

主要成果:

该策略在低负载率 (0.20.8公斤N/m3d) 下有效地积累了酸盐.
缺少碳源 (ACS) 显著上调 narG 和 narI/V 基因表达 (分别为2.5 和 5.1 倍).
塔乌拉被确定为主要的脱剂 (50.2%),对narG和narI/V表达有显著的贡献.
在ACS期间,细胞外糖 (糖,酸盐,葡萄糖) 在关键脱剂中促进了碳中心代谢途径.

结论:

交替的盛宴-饥荒策略通过选择性地增强关键的脱基因,有效地调节部分脱.
这种方法支持丰富特定的功能性细菌,如Thauera,对于酸盐积累至关重要.
这些发现为实际废水处理中酸盐调节提供了工程上可行的和具有成本效益的解决方案.

关键词:

碳节食和饥荒的情况.不化基因调节的基因调节细胞外的代谢产物亚酸盐的积累亚酸盐的积累转录基因机制转录基因机制

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