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修改内细胞网膜形态使得Saccharomyces cerevisiae中的复合抗体表达得到改善

Laura R K Niemelä¹, Essi V Koskela¹, Alexander D Frey¹

¹Aalto University, Department of Bioproducts and Biosystems, Espoo, Finland.

Journal of biotechnology

|March 30, 2024

概括

通过删除网膜基因来修改酵母体内质网膜 (ER) 结构,可显著提高重组蛋白质的产生. 扩大ER光线进一步增强了抗体产量,证明了合成生物学.

科学领域:

合成生物学合成生物学
细胞工程细胞工程
生物技术是生物技术.

背景情况:

麦芽是一种生产重组蛋白质的关键细胞工厂.
蛋白质在细胞内膜网 (ER) 中折叠是生产过程中的一个速度限制的步骤.
ER形态由Rtn1p,Rtn2p和Yop1p等蛋白调节,影响细胞生产力.

研究的目的:

研究ER结构对Saccharomyces cerevisiae细胞生产力的影响.
为了确定操纵ER形态是否可以增强重组蛋白 (抗体) 生产.

主要方法:

创建了酵母菌株,其中删除了编码网膜蛋白质 (Rtn1p,Rtn2p) 和Yop1p的基因.
使用了具有正常或扩展ER光线 (通过OPI1删除) 的菌株.
量化抗体标位,ER表面积,细胞截面积和未折叠的蛋白质反应.

主要成果:

特定酵母菌株的抗体定位比对照高2.2倍.
通过OPI1删除实现的扩展ER膜对于提高生产率至关重要.
扩大ER表面积 (高达2倍) 和细胞大小 (1.5倍) 与提高产量相关.
修改后的菌株显示出减弱的未折叠蛋白质反应.

结论:

在酵母中,ER结构显著影响了细胞生产力.
重编程亚细胞结构,特别是ER形态,是提高生物制造的可行策略.
这种方法整合了合成生物学原理,以改善生物技术应用.

关键词:

细胞内膜网膜是什么细胞内膜网膜再组合蛋白质的表达方式网球回形体网球回形体网球回形体这种植物是Saccharomyces cerevisiae.

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