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实际部署自动化,以加快水性双相提取的速度

Mario A Torres-Acosta¹, Alex Olivares-Molina², Ross Kent³

¹The Advanced Centre for Biochemical Engineering, Department of Biochemical Engineering, University College London, London WC1E 6BT, United Kingdom; Tecnologico de Monterrey, School of Engineering and Science, Av. Eugenio Garza Sada 2501 Sur, Monterrey, N.L. 64849, México.

Journal of biotechnology

|March 30, 2024

概括

本研究介绍了水性双相提取 (ATPE) 工艺开发的自动化框架,提高了粘性生物处理流体的准确性和精度. 该自动化系统简化了开发,以实现更广泛的工业采用.

科学领域:

生物化学工程生物化学工程
分离科学分离科学
过程开发过程开发

背景情况:

水性双相提取 (ATPE) 是一种集成生物处理技术,用于生物质的去除和产品的净化.
由于不可预测的分区和所需的聚合物溶液的高粘度,ATPE的工艺开发具有挑战性.
现有的液体处理设备没有针对ATPE典型的高粘度液体进行优化.

研究的目的:

为ATPE过程开发开发开发一个自动化,高通量框架.
构建相位图并确定各种聚乙烯糖醇 (PEG) 度的双节曲线.
建立粘度和体积独立的传递参数模型,以提高ATPE的准确性和精度.

主要方法:

为PEG6000,PEG3000和PEG2000构建相图并识别双节曲线.
开发模型以确定粘度和体积独立的转移参数.
利用自动化液体处理设备来实现和优化ATPE过程.

主要成果:

自动化框架成功生成了相位图,并确定了双节曲线.
模型准确地预测了粘度和体积独立的转移参数.
使用框架优化ATPE操作显著提高了PEG6000的工艺精度 (平均绝对误差减少6倍) 和设备精度 (变化系数减少3倍).

结论:

自动化液体处理设备可以有效地适应高粘度ATPE应用.
开发的框架为ATPE流程开发提供了一个精确和准确的战略.
这种方法可以简化ATPE的开发,促进其在大规模生物处理中更广泛地采用.

关键词:

水性双相提取方式自动化自动化自动化自动化自动化双极形曲线的双极形曲线液体处理设备是液体处理设备.优化优化优化阶段图阶段图阶段图过程开发过程开发

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