相关实验视频

Updated: Jun 29, 2025

Preparation of Myeloid Derived Suppressor Cells MDSC from Naive and Pancreatic Tumor-bearing Mice using Flow Cytometry and Automated Magnetic Activated Cell Sorting AutoMACS

Preparation of Myeloid Derived Suppressor Cells MDSC from Naive and Pancreatic Tumor-bearing Mice using Flow Cytometry and Automated Magnetic Activated Cell Sorting AutoMACS

Published on: June 18, 2012

通过流式细胞计量检测来自髓质细胞的抑制细胞

Tianju Liu¹, Alyssa Rosek¹, Francina Gonzalez De Los Santos¹

¹Department of Pathology, University of Michigan Medical School, Ann Arbor, MI, United States.

Methods in cell biology

|March 30, 2024

概括

骨髓衍生抑制细胞 (MDSC) 是疾病中的关键免疫抑制细胞. 一个新的流细胞计量协议允许在血液和组织中准确识别和量化它们.

科学领域:

免疫学免疫学免疫学
细胞生物学细胞生物学

背景情况:

骨髓原抑制细胞 (MDSC) 是异质的免疫抑制细胞,在瘤微环境和其他慢性疾病,如感染和炎症中至关重要.
它们的扩散与疾病进展和不良预后相关,表明它们作为预后因素和治疗点的潜力.

研究的目的:

开发一种可靠的方法来识别和量化循环中的髓质衍生抑制细胞 (MDSCs).
建立流细胞计作为在临床和研究环境中评估MDSC的工具.

主要方法:

利用多色光结合抗体对抗表面标记物进行流量细胞计分析.
将该协议应用于人类和小鼠的全血白细胞,外周血液单核细胞和固体组织单细胞悬浮体.

主要成果:

开发了一种流细胞计量协议,用于识别和列举MDSCs.
证明了流细胞计在分析MDSCs复杂表型方面的实用性.

结论:

流细胞计为精确识别和量化MDSCs提供了一种强大的方法.
这种方法有可能成为诊断和治疗结果评估的临床有用工具.

关键词:

流动细胞计量流动细胞计量来自骨髓细胞的抑制细胞.这是PBMC的PBMC.这是全血,全血.

更多相关视频

Flow Cytometry to Estimate Leukemia Stem Cells in Primary Acute Myeloid Leukemia and in Patient-derived-xenografts, at Diagnosis and Follow Up

Flow Cytometry to Estimate Leukemia Stem Cells in Primary Acute Myeloid Leukemia and in Patient-derived-xenografts, at Diagnosis and Follow Up

Published on: March 26, 2018

Two Flow Cytometric Approaches of NKG2D Ligand Surface Detection to Distinguish Stem Cells from Bulk Subpopulations in Acute Myeloid Leukemia

Two Flow Cytometric Approaches of NKG2D Ligand Surface Detection to Distinguish Stem Cells from Bulk Subpopulations in Acute Myeloid Leukemia

Published on: February 21, 2021

相关概念视频

Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...