Search research articles

关于 JoVE

概览领导团队博客 JoVE 帮助中心

作者

出版流程编辑委员会范围与政策同行评审常见问题投稿

图书馆员

用户评价订阅访问资源图书馆顾问委员会常见问题

研究

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments 存档

教育

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual 教师资源中心教师网站

使用条款与条件

相关概念视频

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序

Same author

Long-term stability of autoantibody-based serological profiles in systemic lupus erythematosus.

Reumatologia clinica·2026

Same author

The impact of antinuclear antibodies and complement levels in the prognosis of pregnant women within the antiphospholipid syndrome spectrum.

Frontiers in immunology·2026

Same author

Diagnostic performance of plasma GFAP in preclinical stages of Alzheimer's disease using the Lumipulse platform.

Alzheimer's research & therapy·2026

Same author

Evaluation of IVIG for Prevention of Infections Occurring On-study and Treatment of Infection of Solid Organ Recipients With Secondary Antibody Deficiency: A Phase 2 Randomized Clinical Trial.

Transplantation direct·2026

Same author

Incidence and clinical features of ANCA-associated vasculitis before and during the COVID-19 pandemic: experience from a single-center in Northern Spain.

Frontiers in medicine·2026

Same author

Clinical and Ultrasound Remission in Rheumatoid Arthritis Patients Treated with JAK Inhibitors: A Real-World Study.

Journal of clinical medicine·2026

Search research articles

相关实验视频

Updated: Jun 29, 2025

Real Time Detection of In Vitro Tumor Cell Apoptosis Induced by CD8+ T Cells to Study Immune Suppressive Functions of Tumor-infiltrating Myeloid Cells

Real Time Detection of In Vitro Tumor Cell Apoptosis Induced by CD8+ T Cells to Study Immune Suppressive Functions of Tumor-infiltrating Myeloid Cells

Published on: January 29, 2019

CD4+ T 细胞增殖试验用于分析Mo-MDSCs抑制功能.

María Iglesias-Escudero¹, David San Segundo², Marcos López-Hoyos³

¹Immunology Department, Universitary Hospital Germans Trias i Pujol, Badalona, Spain.

Methods in cell biology

|March 30, 2024

概括

这项研究详细介绍了一项评估髓质衍生抑制细胞 (MDSC) 和它们抑制T细胞增殖的能力的协议. 这种方法有助于理解MDSC在免疫调节和疾病中的功能.

关键词:

CD4 T 细胞是 CD4 的 T 细胞.来自骨髓细胞的抑制细胞.核武器的扩散压制压制是一种压制.

更多相关视频

Assessment of the Immunomodulatory Properties of Human Mesenchymal Stem Cells MSCs

Assessment of the Immunomodulatory Properties of Human Mesenchymal Stem Cells MSCs

Published on: December 24, 2015

Phenotypic and Functional Analysis of Activated Regulatory T Cells Isolated from Chronic Lymphocytic Choriomeningitis Virus-infected Mice

Phenotypic and Functional Analysis of Activated Regulatory T Cells Isolated from Chronic Lymphocytic Choriomeningitis Virus-infected Mice

Published on: June 22, 2016

相关实验视频

Last Updated: Jun 29, 2025

Real Time Detection of In Vitro Tumor Cell Apoptosis Induced by CD8+ T Cells to Study Immune Suppressive Functions of Tumor-infiltrating Myeloid Cells

Real Time Detection of In Vitro Tumor Cell Apoptosis Induced by CD8+ T Cells to Study Immune Suppressive Functions of Tumor-infiltrating Myeloid Cells

Published on: January 29, 2019

Assessment of the Immunomodulatory Properties of Human Mesenchymal Stem Cells MSCs

Assessment of the Immunomodulatory Properties of Human Mesenchymal Stem Cells MSCs

Published on: December 24, 2015

Phenotypic and Functional Analysis of Activated Regulatory T Cells Isolated from Chronic Lymphocytic Choriomeningitis Virus-infected Mice

Phenotypic and Functional Analysis of Activated Regulatory T Cells Isolated from Chronic Lymphocytic Choriomeningitis Virus-infected Mice

Published on: June 22, 2016

科学领域:

免疫学免疫学免疫学
细胞生物学细胞生物学

背景情况:

骨髓调节细胞 (MRC) 包括骨髓衍生抑制细胞 (MDSC),它们调节免疫反应,并与慢性疾病和癌症有关.
将MDSC与其他髓状细胞区分开来是具有挑战性的,因为缺乏特定的标记物,需要进行功能性检测.

研究的目的:

为评估人类单细胞MDSCs (Mo-MDSCs) 的T细胞抑制功能提供详细的体外协议.
通过分析它们对T淋巴细胞增殖的影响,使Mo-MDSCs的功能性表征成为可能.

主要方法:

从周围血液中分离Mo-MDSC和CD4+T细胞.
在Mo-MDSCs与CD4+T细胞的体外共培.
对Mo-MDSC共同培养的反应中T细胞增殖的评估.

主要成果:

该协议允许评估Mo-MDSC介导的T细胞增殖抑制.
功能评估为Mo-MDSCs的监管能力提供了洞察力.

结论:

该协议提供了一种标准化方法,用于对人类Mo-MDSCs进行功能性表征.
了解MDSC抑制活性对于癌症免疫学和慢性炎症疾病的研究至关重要.