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评估骨髓系衍生抑制细胞分化在活体中

Ester Blanco¹, David Escors², Grazyna Kochan²

¹Oncoimmunology Research Unit, Navarrabiomed-Fundación Miguel Servet, Hospital Universitario de Navarra (HUN), Instituto de Investigación Sanitaria de Navarra (IdiSNA), Pamplona, Spain; Division of Gene Therapy and Regulation of Gene Expression, Cima Universidad de Navarra and Instituto de Investigación Sanitaria de Navarra (IdISNA), Pamplona, Spain.

Methods in cell biology

|March 30, 2024

概括

骨髓衍生抑制细胞 (MDSCs) 是癌症进展的关键驱动因素. 这项研究提出了一种新的ex vivo分化方法,用于为癌症研究和药物开发生成可靠的MDSC.

科学领域:

免疫学免疫学免疫学
癌症生物学癌症生物学
细胞生物学细胞生物学

背景情况:

骨髓系衍生抑制细胞 (MDSCs) 通过抑制抗瘤免疫力来促进癌症的进展和转移.
通过T细胞失活和细胞因子产生,MDSCs通过T细胞失活和细胞因子产生免疫抑制瘤微环境 (TME).
鉴定和净化瘤透的MDSCs是具有挑战性的,因为它们与常规髓状细胞的相似性和数量低.

研究的目的:

建立一种可靠的方法,以实体分化髓状细胞衍生抑制细胞 (MDSCs).
为了生成MDSC,准确地模拟瘤透对应物用于癌症研究.
通过实现高产量的MDSC净化,促进高通量药物验证试验.

主要方法:

设计一种小鼠瘤细胞系,以构成性地表达粒细胞单细胞群刺激因子 (GM-CSF).
从工程细胞系收集条件介质,以诱导MDSC与骨髓前体的分化.
隔离骨髓细胞并应用一种特殊的协议来进行ex vivoMDSC分化.

主要成果:

活体差异化方法产生了MDSC子集,对自然瘤透的MDSC具有很高的忠实性.
该协议允许对差异化的MDSC进行高产量的净化.
生成的MDSC密切模仿其体内对应物.

结论:

该协议提供了一种可靠的方法来生成功能性的MDSCsex vivo.
这些细胞的高产量和忠实性支持它们在验证新型癌症疗法的使用.
这种方法克服了研究MDSC的挑战,推动了癌症免疫学研究.

关键词:

在活体外生成的MDSC.骨髓系衍生抑制细胞 (MDSCs) 是一种瘤微环境 (TME) 是指瘤的微环境.

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