治疗性血交换对完整的蛋白S,阿波利波蛋白和血栓生成的影响
Hanna H Pitkänen1, Mikko Haapio2, Mika Saarela3
1Helsinki University, Division of Anesthesiology, Department of Anesthesiology, Intensive Care and Pain Medicine, University of Helsinki and Helsinki University Hospital, Helsinki, Finland; Department of Hematology, Coagulation Disorders Unit, Helsinki University Hospital, Helsinki, Finland, and Research Program in Systems Oncology, Faculty of Medicine, University of Helsinki, Finland.
概括
治疗性血交换 (TPE) 与S/D血改变了脂质平衡和凝血. 降低完整的蛋白S (PS) 和阿波利波蛋白表明在体内血静和脂质资料的再平衡.
科学领域:
- 身体之外的血液净化
- 血液静止和血栓形成
- 脂质代谢 脂质代谢是什么
背景情况:
- 使用溶剂/洗剂 (S/D) 处理的治疗性血交换 (TPE) 影响凝血和脂质.
- 以前的体外研究表明,S/D-血通过减少完整的蛋白S (PS) 来增强血栓生成.
研究的目的:
- 评估改变脂质平衡对TPE期间与S/D血对凝血的影响.
- 为了确定体外观察到的降低完整PS水平和前凝性表型是否发生在体内.
主要方法:
- 凝血生物标志物,血栓生成 (校准自动血栓图 - CAT) 和脂质水平在接受TPE的6名患者中被测量.
- 在实验室中,S/D-血的稀释被用于模仿TPE对血栓生成的影响.
主要成果:
- 带有S/D血的TPE导致完整的PS,LDL,Apolipoprotein CIII (ApoC3) 和ApoB/ApoA1比率下降.
- 血凝素生成显示了缩短的滞后时间,表明了前凝剂的转移.
- 在试验室稀释中部分缓解了S/D-血的前凝性表型.
结论:
- 使用S/D-血的TPE,结合肝素化,会影响静血和脂质状况.
- 完整的PS和apolipoproteins的下降表明这些特征在体内重新平衡.
- 该研究提供了体内证据,证明TPE期间凝血和脂质动态发生变化.
更多相关视频
相关概念视频
01:30Anticoagulant Drugs: Low-Molecular-Weight Heparins
690
Hemostasis is a crucial process that prevents excessive blood loss from damaged blood vessels. It involves various mechanisms such as vasoconstriction, platelet adhesion and activation, and fibrin formation. The importance of each mechanism depends on the type of vessel injury. In contrast, thrombosis is the abnormal formation of a blood clot within the blood vessels, leading to potential complications if the clot obstructs blood flow. Thrombosis can be caused by increased coagulability of the...
690
01:16Clot Retraction and Fibrinolysis
5.8K
After a fibrin clot is formed, the next step is clot retraction, a vital process facilitated by platelet contractile proteins, such as actin and myosin. These proteins pull the fibrin strands closer together and condense the clot. This action reduces the size of the clot, creating a smaller, denser structure that effectively seals off the damaged vessel. Clot retraction consolidates the clot and helps with wound healing by bringing the edges of the damaged blood vessel closer together.
5.8K
01:29Drug Distribution: Plasma Protein Binding
5.6K
Drugs predominantly attach to plasma proteins, with only a small percentage remaining unbound. The unbound portion can be calculated as one minus the bound fraction. Acidic drugs form large, inactive complexes by reversibly binding to plasma albumin, which prevents them from diffusing across biological barriers. These drug-protein complexes act as reservoirs for the drugs. As the concentration of unbound drugs decreases, these complexes quickly dissociate to release the free drug, maintaining...
5.6K
01:22Formation of the Platelet Plug
6.1K
The platelet phase, the second stage of hemostasis, commences around 15-20 seconds after an injury. It follows and overlaps with the vascular phase, during which blood vessels constrict to minimize blood loss.
As the injured blood vessel contracts, endothelial cells undergo contraction, revealing collagen fibers in the basement membrane and underlying connective tissue. Furthermore, the plasma membrane of endothelial cells becomes adhesive, preparing the site for platelet adhesion. Platelets...
As the injured blood vessel contracts, endothelial cells undergo contraction, revealing collagen fibers in the basement membrane and underlying connective tissue. Furthermore, the plasma membrane of endothelial cells becomes adhesive, preparing the site for platelet adhesion. Platelets...
6.1K
01:23Factors Affecting Protein-Drug Binding: Drug Interactions
139
Drug interactions are a critical aspect of pharmacology and can occur when two or more drugs compete for the same binding site. This competition can result in one drug displacing another, altering the effect of the displaced drug. Drug interactions are complex processes that rely heavily on how much of the displacer drug is present and how strongly it can bind to the same sites as the displaced drug.
Displacement interactions can have varying outcomes, ranging from toxicity to virtually...
Displacement interactions can have varying outcomes, ranging from toxicity to virtually...
139
01:20Extrinsic and Intrinsic Pathways of Hemostasis
7.5K
Blood clotting or coagulation involves extrinsic and intrinsic pathways, which ultimately merge into the common pathway, forming a fibrin clot.
The Extrinsic Pathway
The extrinsic pathway of coagulation is typically initiated by tissue damage that exposes blood to tissue factor (TF), a protein released by the damaged tissue cells outside the blood vessels—this interaction with TF triggers biochemical reactions involving specific clotting factors. The key player here is Factor VII, which...
The Extrinsic Pathway
The extrinsic pathway of coagulation is typically initiated by tissue damage that exposes blood to tissue factor (TF), a protein released by the damaged tissue cells outside the blood vessels—this interaction with TF triggers biochemical reactions involving specific clotting factors. The key player here is Factor VII, which...
7.5K