揭开肥胖和维生素D代谢之间的复杂相互作用
Bashar Alzohily1, Asma AlMenhali2, Salah Gariballa3
1Department of Chemistry, College of Science, UAE University, 15551, Al Ain, UAE.
Scientific reports
|March 30, 2024
概括
肥胖与低维生素D水平有关. 一种新的UHPLC-MS/MS方法准确地测量维生素D代谢物,即使在补充剂后也能揭示缺乏,突出显示肥胖个体的代谢挑战.
科学领域:
- 临床化学 临床化学
- 营养科学 营养科学
- 质谱测量质量谱测量
背景情况:
- 维生素D缺乏和肥胖是全球重要的健康问题.
- 肥胖症使维生素D状态的评估复杂化,原因是维生素D的分布和吸收发生了改变.
- 准确测量维生素D代谢物对于了解这些健康问题至关重要.
研究的目的:
- 开发和验证一种超高性能液体染色学-并联质谱法 (UHPLC-MS/MS) 方法,用于分析血清中的维生素D代谢物.
- 评估肥胖个体 (缺乏和补充) 和健康志愿者的维生素D代谢物水平.
主要方法:
- 分析了来自452名受试者的血清样本 (缺乏肥胖,补充肥胖,健康).
- 开发并验证了一种UHPLC-MS/MS方法,用于分离和量化维生素D代谢物.
- 在不同受试者群体中比较维生素D和代谢物度.
主要成果:
- 通过UHPLC-MS/MS方法证明了足够的分离,并满足了验证标准.
- 所有组,包括健康的志愿者,都表现出25-氧维生素D2和25-氧维生素D3的缺乏.
- 补充的肥胖患者显示维生素D3和25-氧维生素D3的含量增加,但水平仍然低于最佳水平.
结论:
- 经过验证的UHPLC-MS/MS方法对于测量维生素D代谢物是有效的.
- 肥胖个体,即使补充,经常表现出持续的维生素D缺乏.
- 肥胖的代谢和储存效应显著影响维生素D状态的评估.
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