人类血液型A脏的酶转化为通用血液型O
Serena MacMillan1, Sarah A Hosgood2, Léonie Walker-Panse2
1Department of Surgery, University of Cambridge, Cambridge, UK. sgmjm2@cam.ac.uk.
Nature communications
|March 30, 2024
概括
来自Flavonifractor plautii的酶将A型血液抗原转化为通用O型抗原. 这种ABO兼容的方法减少了抗A抗体结合和补体激活,解决了供体器官短缺问题.
科学领域:
- 生物化学 生物化学
- 移植免疫学 移植免疫学
- 微生物学 微生物学
背景情况:
- ABO血型不相容是移植的一个主要限制.
- 克服 ABO 障碍的现有策略是有限的.
研究的目的:
- 为了酶性地修改人类脏血管系统,以消除A血型抗原.
- 在ex vivo perfusion模型中评估酶性ABO抗原转换的有效性.
主要方法:
- 使用了来自Flavonifractor plautii的FpGalNAc脱乙酸酶和FpGalactosaminidase.
- 采用常温机器输液 (NMP) 和低温机器输液 (HMP) 进行酶处理.
- 通过测量ex vivo模型中的抗A抗体结合和补体激活来评估抗原转化.
主要成果:
- 在NMP和HMP的2小时内,血液A组抗原减少了大约80%.
- 接受治疗的脏没有结合循环中的抗A抗体.
- 在接受治疗的脏中,证明了经典补充通路激活的废除.
结论:
- 血型A抗原的酶转化为ABO兼容移植提供了一个有前途的策略.
- 这种方法有可能显著增加捐赠器官的可用性,并减少患者的等待时间.
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