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联合CovRS双组分调控系统与通过克林达米辛治疗的纳达酶诱导在Streptococcus pyogenes中

Ichiro Tatsuno¹, Masanori Isaka¹, Tadao Hasegawa¹

¹Department of Bacteriology, Nagoya City University Graduate School of Medical Sciences, Japan.

Japanese journal of infectious diseases

|March 31, 2024

概括

高剂量克林达米辛 (CLI) 治疗可以诱导Streptococcus pyogenes中的NAD-糖酶 (NADase). 该研究发现,CovS蛋白对于这种依赖克林达米辛的NADase诱导至关重要,独立于SpeB.

科学领域:

微生物学微生物学
分子生物学分子生物学
毒理学毒理学毒理学

背景情况:

链球菌毒性休克综合征 (STSS) 用高剂量的克林达米辛 (CLI) 和青素治疗.
全球范围内正在出现抗CLI的Streptococcus pyogenes菌株.
在某些菌株中,CLI治疗可以增加NAD-糖酶 (NADase) 外毒素活性.

研究的目的:

为了研究在Streptococcus pyogenes中依赖CLI的NADase诱导背后的机制.
为了确定与这种现象有关的特定遗传因素.

主要方法:

使用了13种由CLI敏感性Streptococcus pyogenes1529菌株衍生而来的两组分传感器淘汰菌株.
在这些淘汰菌株中评估了依赖CLI的NADase诱导表型.
具体研究了CovS,SpeB,Maga和Rgg的作用.

主要成果:

对CLI敏感和对CLI耐药的Streptococcus pyogenes菌株表现出依赖于CLI的NADase诱导.
只有1529ΔcovS淘汰菌株失去了CLI-依赖的NADase诱导表型.
淘汰杆菌株1529ΔspeB,1529Δmga和1529Δrgg保留了该表型.

结论:

两组分传感器CovS对于在Streptococcus pyogenes中依赖于CLI的NADase诱导至关重要.
这种诱导途径的调节由CovS独立于SpeB进行.
了解这种机制可能会为针对CLI耐药菌株的策略提供信息.

关键词:

纳达酶 NADase 是一个这是一种Streptococcus pyogenes.克林达米辛是一种

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