葡萄糖类-1受体激活剂对小鼠丸和丸衍生细胞系中精子生成相关基因表达的作用
Masashi Iida1,2, Atsushi Asano1
1Laboratory of Laboratory Animal Science, Joint Faculty of Veterinary Medicine, Kagoshima University, Kagoshima, Japan.
The Journal of veterinary medical science
|March 31, 2024
概括
类似葡萄糖-1 (GLP-1) 存在于小鼠丸中,并刺激塞尔托利细胞. 这种可以增强对男性生殖功能和精子生成至关重要的基因的表达.
科学领域:
- 生殖生物学 生殖生物学
- 内分泌学 在内分泌学.
- 分子生物学分子生物学
背景情况:
- 类似葡萄糖的-1 (GLP-1) 是一种内激素,在葡萄糖调节中具有已知的作用.
- 新出现的证据表明,隐形素具有胰腺外作用,可能有利于男性不孕症等疾病.
- GLP-1对男性生殖功能的特定影响仍然在很大程度上未被探索.
研究的目的:
- 研究GLP-1受体在雄性小鼠生殖系统中的表达.
- 确定GLP-1对丸细胞中与精子发生相关的基因的影响.
- 阐明GLP-1在男性生殖健康中的潜在作用.
主要方法:
- 在小鼠丸和相关细胞系 (Sertoli,Leydig,生殖细胞) 中分析 Glp1r mRNA和GLP-1蛋白表达.
- 用GLP-1受体agonist exenatide治疗小鼠塞尔托利 (TM4) 细胞和雄性小鼠.
- 评估与精子发生有关的基因 (Kitl,Pdgfa) 和Glp1r) 的mRNA表达水平,在施用exenatide后.
- 在治疗细胞激活剂后,包括布克拉迪辛在内的TM4细胞中评估Glp1r mRNA表达.
主要成果:
- 在小鼠丸中检测到GLP-1受体 (Glp1r) mRNA和GLP-1蛋白,其水平与脂肪组织,肝脏和肠道的水平相似或超过.
- 埃克塞纳提德治疗显著提高了TM4塞尔托利细胞中的Kitl,Pdgfa和Glp1rmRNA的调节.
- 在体内给予雄性小鼠的exenatide导致了Kitl和Glp1rmRNA的显著增加,并趋向于增加PdgfamRNA.
- 布克拉丁治疗显著增加了TM4细胞中的Glp1r mRNA表达.
结论:
- GLP-1在小鼠丸中表达,并对塞尔托利细胞产生急性作用.
- GLP-1信号刺激了参与精子生成的关键基因的表达.
- 这些发现表明GLP-1在调节男性生殖功能方面可能发挥作用.
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