在日本从野猪中检测和隔离了基因型3亚型b肝炎E病毒
Wenjing Zhang1, Yen Hai Doan2, Tian-Cheng Li1
1Department of Virology II, National Institute of Infectious Diseases, Tokyo, Japan.
The Journal of veterinary medical science
|March 31, 2024
概括
肝炎E病毒基因型3亚型b (HEV-3b) 在日本野猪中普遍存在. 这项研究强调了从动物样本直接测序对于理解HEV流行病学的重要性.
科学领域:
- 兽医病毒学 兽医病毒学
- 流行病学 流行病学
- 分子生物学分子生物学
背景情况:
- 肝炎E病毒 (HEV) 是一种新兴的动物性传染病原体.
- 野猪是已知的HEV储库,对人类构成潜在的风险.
- 了解野生动物群体中的HEV循环对于公共卫生至关重要.
研究的目的:
- 调查日本野猪中HEV的流行率和遗传特征.
- 为了确定这种野生动物群体中流通的HEV基因型和亚型.
- 评估来自野猪的HEV传播潜力.
主要方法:
- 从日本八个县的野猪收集血清和便样本.
- 使用血清分析和RT-qPCR检测抗HEV IgG抗体和HEV RNA.
- 检测到的HEV菌株 (ORF2和完整基因组) 的部分和全基因组测序.
- 使用PLC/PRF/5细胞进行病毒隔离和细胞培养.
主要成果:
- 在血清样本中,抗HEV IgG抗体的患病率为21.8%.
- 在6.1%的血清和3.1%的便样本中检测到HEVRNA.
- 所有测序的HEV菌株都属于基因型3亚型b (HEV-3b).
- 测序的HEV-3b菌株显示出遗传多样性,但聚集在一起.
- 确定了HEV-3b菌株WB0567的完整基因组,在复制过程中发现了11个核酸突变.
结论:
- 在日本各地的野猪中,HEV-3b在野猪中均传播.
- 野猪是HEV-3b.的重要储存库.
- 动物样本的直接测序对于准确的HEV序列确定和流行病学分析至关重要.
- 需要进一步的研究来评估这些HEV菌株的动物感染潜力.
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