在金属氧化物上的蛋白质聚合控制着催化活动和细胞吸收
Robert Nißler1,2,3, Lena Dennebouy1, Alexander Gogos1,2
1Nanoparticle Systems Engineering Laboratory, Institute of Energy and Process Engineering (IEPE), Department of Mechanical and Process Engineering (D-MAVT), ETH Zurich, Sonneggstrasse 3, Zurich, 8092, Switzerland.
Small (Weinheim an der Bergstrasse, Germany)
|March 31, 2024
概括
金属氧化物纳米颗粒上的蛋白质冠状会影响它们在生物系统中的催化活性. 了解这种蛋白质层有助于优化纳米催化剂设计用于生物医学应用.
科学领域:
- 生物医学工程 生物医学工程
- 纳米技术纳米技术
- 材料科学 材料科学 材料科学
背景情况:
- 催化活性无机纳米材料,特别是金属氧化物,具有多种生物医学应用,包括伤口愈合和癌症治疗.
- 在生物暴露后,纳米材料获得蛋白质冠状,影响它们的相互作用和有效性.
- 蛋白冠对金属氧化物纳米催化剂的催化活性的影响仍然不太清楚.
研究的目的:
- 为了研究金属氧化物纳米粒子上的蛋白冠的组成.
- 为了将蛋白质吸附与这些纳米材料的催化活性在体外和体内相关联.
- 阐明蛋白冠如何调节生物环境中的纳米催化剂性能.
主要方法:
- 蛋白质组分析以确定吸附的蛋白质.
- 纳米分析技术来表征蛋白质层.
- 在体外和体内测试以评估纳米催化剂活性.
- 评估蛋白质冠状体降解及其对活动的影响.
主要成果:
- 蛋白冠可以包括多达280种蛋白质,占纳米粒子重量的38%.
- 冠状元中的opsonin促进了巨细胞的吸收,在巨细胞中定位了>99%的局部纳米催化剂.
- 最初的蛋白质冠状形成显著降低了纳米催化剂活性,该活性在内体条件下通过蛋白质分解性降解被部分恢复.
结论:
- 蛋白冠极大地影响金属氧化物纳米粒子的催化活性.
- 蛋白冠的蛋白质分解可以部分恢复纳米催化剂的功能.
- 这些发现为开发复杂生物应用的有效纳米催化剂提供了关键的设计参数.
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