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精密工程对酶的协同固定用于级联生物催化剂的工程

Zhiyuan Luo¹, Li Qiao¹, Haomin Chen¹

¹College of Material, Chemistry and Chemical Engineering, Hangzhou Normal University, China, Hangzhou, Zhejiang, 311121, China.

Angewandte Chemie (International ed. in English)

|April 1, 2024

概括

研究人员开发了一种新的双酶固定化技术,使用生物对角化学. 这种方法有效地合成了高纯度的奇拉醇,并提高了级联生物催化剂的酶载荷能力.

科学领域:

生物催化和酶工程生物催化和酶工程
生物技术是生物技术.
化学合成化学合成

背景情况:

在固体支上对多种酶的联合固定对于级联生物催化剂至关重要,但存在重大设计挑战.
开发有序,分层的酶固定化策略对于高效的多步骤酶反应至关重要.

研究的目的:

开发一种新的方法,可以有序地,分层地将多种酶联合固定在树脂颗粒上.
创建一个双酶反应堆,用于不对称的合成和现场辅因子再生.

主要方法:

带有SpyTag/SpyCatcher域的工程性酒精脱酶 (ADH) 和基因减少酶 (AKR) 酶.
基因结合的非正规氨基酸 (ncAA) 用于共价酶定.
使用SpyTag/SpyCatcher和亚酸循环添加剂对微球进行连续的双酶涂层.

主要成果:

在多孔的微球上实现了有序的双酶涂层,使 (S) - 1 - 2 - 烯基乙醇与99.9%的反体过剩 (ee) 的不对称合成成为可能.
双酶反应器显示了74%的催化转化,在六个循环后保持了80%的活性,并且与单层方法相比显示了1.7倍以上的酶负载能力.
成功实现了多个酶的同时净化和固定,促进了级联生物催化.

结论:

开发的顺序双酶固定化策略为级联生物催化剂提供了方便和高效的方法.
这种方法显著增强了酶负载,并在多个循环中保持高产品纯度和酶活性.
为各种合成应用构建复杂的酶系统提供了多功能平台.

关键词:

不对称的减值不对称的减少生物的正交性是生物的正交性.酶级联是一种酶级联.命令不动化的命令.

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