在先天感染的胚胎和幼虫中,微型菌的增殖和宿主反应的转录组变化
Zigang Shen1,2,3, Zhuojun Ke2,3, Qiong Yang4
1College of Sericulture, Textile and Biomass Sciences, Southwest University, Tiansheng Street, Chongqing, 400716, China.
BMC genomics
|April 1, 2024
概括
先天性Nosema bombycis感染会影响丝虫,改变寄生虫的新陈代谢和宿主免疫反应. 这项研究揭示了感染进展期间宿主和病原体的关键基因表达变化.
科学领域:
- * 分子生物学 * 分子生物学
- * 寄生虫学 寄生虫学
- * 基因组学 是一个学科.
背景情况:
- *先天性微型虫 (Nosema bombycis) 感染在丝虫 (Bombyx mori) 养殖中造成重大经济损失.
- *以前的研究集中在非编码RNA,使得寄生虫繁殖和宿主反应在先天性感染期间的转录基因转移不清楚.
研究的目的:
- * 在先天性感染期间同时分析N. bombycis和B. mori的转录组.
- *阐明微型菌体的增殖和宿主防御策略背后的分子机制.
主要方法:
- * 在先天性感染期间对N. bombycis和B. mori胚胎 (5天) 和幼虫 (1,5-10天) 的全转录组测序.
- *使用Venn图和热图进行差异基因表达分析.
- *使用RT-qPCR验证关键差异表达基因 (DEGs).
主要成果:
- * N. bombycis与中央碳代谢相关的基因在幼虫中被下调,而增殖/生长基因被上调. 确定了关键的与感染相关的基因 (Ricin B lectin,子壁蛋白).
- * 随着感染的进展,B. mori DEGs包括DNA复制,mRNA监测,蛋白质生物合成,特别是免疫通路 (细胞分裂,亡,TNF,Toll-like受体,NF-kappa B) 的 DEGs.
- * 与胰岛素信号和脂质代谢相关的宿主基因在幼虫中被上调. 表观遗传调节剂 (多,脱甲基酶,甲基转移酶) 也被确定为DEGs.
结论:
- * 同时的转录基因分析提供了对N. bombycis先天性感染期间宿主-寄生虫相互作用的全面了解.
- *这项研究强调了寄生虫毒性因素和宿主免疫反应的动态变化,为丝虫疾病管理提供了洞察力.
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