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脱细胞化大脑细胞外矩阵水凝有助于人类脊髓器官的形成,概括复杂的三维组织

Weidong Wu^1,2,3, Youjun Liu^2,3, Renfeng Liu^2,3

¹Department of Orthopedics, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, China.

ACS biomaterials science & engineering

|April 1, 2024

概括

研究人员从老鼠大脑细胞外基质开发了一种新的水凝,用于培养人类脊髓器官. 这种更安全,更相关的材料改善了脊髓器官的发育,用于疾病建模和药物发现.

科学领域:

干细胞生物学干细胞生物学
神经科学是一个神经科学.
生物材料科学是生物材料的科学.

背景情况:

建立脊髓疾病的体外模型是具有挑战性的,因为组织的复杂性.
目前的体内和体外模型发展不足,阻碍了治疗的发展.
人类诱导的多能干细胞 (hiPSC) 衍生器官具有前景,但Matrigel,一种来自小鼠的矩阵,是标准培养材料.

研究的目的:

开发一种更安全,更生理相关的Matrigel替代品,用于人类脊髓器官培养.
研究大鼠脱细胞化大脑细胞外基质水凝 (DBECMH) 在支持hiPSC衍生脊髓器官的形成和成熟方面的疗效.

主要方法:

制备大鼠脱细胞化大脑细胞外矩阵水凝 (DBECMH).
使用DBECMH.培养hiPSC衍生的脊髓器官.
在DBECMH和Matrigel中比较有机体发育和标记物表达.

主要成果:

DBECMH成功地支持了hiPSC衍生的脊髓器官的形成.
与Matrigel相比,在DBECMH培养的器官显示出来自多个脊髓区的标记物的表达水平较高.
DBECMH促进了脊髓器官组织的增强发育和成熟.

结论:

DBECMH是一种有前途的生物材料,用于培养人类脊髓器官.
这种矩阵有可能取代Matrigel,推进脊髓疾病的体外建模.
这项研究为改进脊髓器官培养方案和治疗应用铺平了道路.

关键词:

脱细胞化的大脑脱细胞化的大脑细胞外矩阵是细胞外矩阵.这种水凝是水凝.脊髓中的有机体

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