开发一种能够逆转多重药物耐药性瘤组合化疗的aptamer
Xiaohong Wen1,2,3, Zhixiang Huang1,3,4, Xiaohai Yang1,2,3
1State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University, Changsha 410082, China.
概括
一种新开发的DNA吸收剂有效地逆转了肝癌细胞中的多药性耐药性. 这种胺体通过增加药物积累来增强化疗效,显示出改善癌症治疗的巨大潜力.
科学领域:
- 生物技术是生物技术.
- 分子生物学分子生物学
- 在瘤学瘤学.
背景情况:
- 多药耐药性 (MDR) 显著限制化疗在治疗各种癌症中的有效性.
- 开发可逆转MDR的药物对于提高治疗结果和克服治疗失败至关重要.
研究的目的:
- 选和表征一种能够在人类肝细胞癌 (HCC) 细胞中逆转MDR的DNA吸收体.
- 评估所选的阿普塔默作为治疗肝癌治疗的治疗剂的潜力.
主要方法:
- 通过指数式丰富 (SELEX) 进行基于细胞的连接体系统演化,用于识别对抗多克索鲁比 (DOX) 耐药的HepG2/DOX细胞的阿帕特马.
- 研究了aptamer的亲和力,特异性和MDR逆转的机制.
- 使用异种移植小鼠模型进行了体内研究,以评估治疗疗效.
主要成果:
- 鉴定出一种特定的DNA胺体,d3,对HepG2/DOX细胞具有很高的亲和力和特异性,向多药耐药性蛋白1 (MDR1).
- 阿普塔默d3证明了其阻断MDR的能力,增加化疗剂 (DOX,温克里斯,帕克利塔塞尔) 的细胞内积累,并增强癌细胞杀死.
- 在体内,阿帕特马尔d3和DOX的联合治疗显著抑制了瘤的生长.
结论:
- 这种DNA的aptamerd3显示出作为一种治疗剂的显著潜力,可以逆转瘤MDR.
- 结合aptamer d3与常规化疗药物,为改善肝癌治疗结果提供了一个有前途的策略.
更多相关视频
08:59Looking for Driver Pathways of Acquired Resistance to Targeted Therapy: Drug Resistant Subclone Generation and Sensitivity Restoring by Gene Knock-down
Published on: December 11, 2017
7.2K
14:20Polymalic Acid-based Nano Biopolymers for Targeting of Multiple Tumor Markers: An Opportunity for Personalized Medicine?
Published on: June 13, 2014
16.7K
相关概念视频
02:56Treatment Resistant Cancers
3.3K
Cancer is the second leading cause of death in the United States. A cancer cell is genetically unstable and hence can mutate faster. They can also modify their microenvironment and escape immune surveillance. The difficulties in treating cancer are further compounded by the emergence of rapid resistance to anticancer drugs. The most common ways to attain resistance in cancer cells include alteration in drug transport and metabolism, modification of drug target, elevated DNA damage response, or...
3.3K
02:57Targeted Cancer Therapies
7.6K
The targeted cancer therapies, also known as “molecular targeted therapies,” take advantage of the molecular and genetic differences between the cancer cells and the normal cells. It needs a thorough understanding of the cancer cells to develop drugs that can target specific molecular aspects that drive the growth, progression, and spread of cancer cells without affecting the growth and survival of other normal cells in the body.
There are several types of targeted therapies against...
There are several types of targeted therapies against...
7.6K
02:50Combination Therapies and Personalized Medicine
4.9K
Combining two or more treatment methods increases the life span of cancer patients while reducing damage to vital organs or tissue from the overuse of a single treatment. Combination therapy also targets different cancer-inducing pathways, thus reducing the chances of developing resistance to treatment.
The combination of the drug acetazolamide and sulforaphane is a good example of combination therapy to treat cancer. The cells in the interior of a large tumor often die due to the hypoxic and...
The combination of the drug acetazolamide and sulforaphane is a good example of combination therapy to treat cancer. The cells in the interior of a large tumor often die due to the hypoxic and...
4.9K