在肠道上皮的 BEST4+ 细胞
Tania Malonga1,2, Nathalie Vialaneix2,3, Martin Beaumont1
1GenPhySE, Université de Toulouse, INRAE, ENVT, 31326, Castanet Tolosan, France.
American journal of physiology. Cell physiology
|April 1, 2024
概括
单细胞转录组学揭示了一种新的人类肠道细胞类型,BEST4阳性细胞,对pH调节和电解质运输至关重要. 由于它们在小鼠中不存在,因此需要使用有机体或其他动物模型进行进一步研究.
科学领域:
- 胃肠病学 胃肠病学
- 细胞生物学 细胞生物学
- 人体生理学 人体生理学
背景情况:
- 单细胞转录组学已经确定了人类肠道上皮质中成熟的吸收细胞的新型血统,被指定为BEST4阳性 (BEST4+) 细胞.
- 这些细胞的特征是表达BEST4和其他标记物,如OTOP2,CA7,GUCA2A,GUCA2B和SPIB.
- 从早期发育开始,BEST4+细胞遍布人类小肠和大肠,占上皮细胞的不到5%.
研究的目的:
- 审查和总结关于人类肠道上皮质中BEST4+细胞的现有文献.
- 描述BEST4+细胞在肠道健康和疾病中的预测作用.
- 突出研究BEST4+细胞的挑战和潜在解决方案,因为它们在小鼠中不存在.
主要方法:
- 对BEST4+细胞的当前科学文献的综述.
- 从单细胞转录组学研究中分析基因表达特征.
- 对研究BEST4+细胞的潜在动物模型和体外系统的评估.
主要成果:
- BEST4+细胞表现出特定位置的基因表达,这表明在不同肠道区域的功能专业化 (例如,小肠中的CFTR表达).
- 预测的功能包括感知/调节光pH值,调整瓜尼环酶-C信号,电解质运输,粘液水化和抗菌分泌.
- 贝斯特4+细胞在人类肠道有机体和几个动物物种 (,猪,斑马鱼,子) 中发现,但在小鼠中没有发现.
结论:
- BEST4+细胞代表了一种独特的吸收性细胞亚型,在肠道平衡中起着关键作用.
- 它们的拟议角色的功能验证需要替代模型,如人类肠道有机体或合适的动物模型 (例如,子).
- 了解BEST4+细胞对于破译肠道功能和疾病至关重要,特别是考虑到它们的独特特征和在小鼠中缺乏.
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