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突变繁殖耐高压菌株,从玉米草中生产酸

Jing Wu¹, Yilian Li¹, Jinbao Yin¹

¹Shanxi Key Laboratory of Chemical Product Engineering, College of Chemical Engineering and Technology, Taiyuan University of Technology, Taiyuan, 030024, China.

Applied microbiology and biotechnology

|April 1, 2024

概括

使用大气和室温等离子体 (ARTP) 突变生成,开发出了一种新的Actinobacillus succinogenes菌株M4. 这种增强的菌株显著增加了113%的玉米炉水解酸的酸生产.

科学领域:

生物技术是生物技术.
微生物工程微生物工程
可持续化学可持续化学

背景情况:

玉米炉是生产酸的可持续原料.
预处理副产品可以抑制微生物发酵.
开发强大的微生物菌株对于高效的生物处理至关重要.

研究的目的:

开发一种抗压力抗菌株的Actinobacillus succinogenes,以提高酸的生产.
为了利用未经排毒的玉米炉水解剂作为发酵基质.
为了确定赋予抗压力和提高生产力的遗传修饰.

主要方法:

大气和室温等离子体 (ARTP) 突变发生被用来产生一种抗压力菌株.
艾克提诺细菌糖原体CICC11014是原始菌株,而A.糖原体M4是突变的衍生物.
进行了全基因组再测序,以确定菌株之间的遗传差异.

主要成果:

与原始菌株相比,A. succinogenes M4的抗压能力增加了两倍.
使用A. succinogenes M4与玉米炉水解剂,木酸的产量增加了113%.
在A. succinogenes M4.中发现了四种非同义基因突变和两种上游基因丢失突变.

结论:

ARTP突变发生是有效的发展耐压力微生物菌株.
A. succinogenes M4 是一个有前途的候选物,可用于从红纤维素生物质中工业生产红酸.
遗传分析提供了对抗压力和提高生产力的机制的见解.

关键词:

亚克蒂诺巴西卢斯 (Actinobacillus succinogenes) 是一种植物.大气中的室温等离子体玉米草的玉米草是一个很好的方法.发酵发酵是一个过程.葡萄糖酸是什么葡萄糖酸是什么

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