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在 fenoxaprop-p-ethyl 压力下,Microcystis aeruginosa 的生理学,抗氧化系统和基因表达的变化

Sijia Liu¹, Jiawei Ni¹, Ying Guan¹

¹School of Chemical and Environmental Engineering, Shanghai Institute of Technology, Shanghai, 201418, China.

Environmental science and pollution research international

|April 1, 2024

概括

氧-p-乙烯 (FE) 除草剂影响微囊菌的生长和毒素的产生. 低度的FE刺激了生长,而高剂量则抑制了生长,并减少了微囊素的产生.

科学领域:

环境毒理学环境毒理学
水生微生物学水生微生物学
生物化学生物化学

背景情况:

氧-p-乙烯 (FE) 是一种广泛使用的除草剂,具有潜在的生态影响.
微囊气球菌的开花对人类健康和水生生态系统构成风险.
关于FE对M. aeruginosa的生理影响的研究有限.

研究的目的:

研究M. aeruginosa对不同FE度的生理和生化反应.
评估FE对M. aeruginosa生长,氧化应激和微囊素-LR (MC-LR) 生产的影响.
分析FE对与光合作用和微囊素合成相关的关键基因表达的影响.

主要方法:

培养M. aeruginosa并将其暴露在不同的FE度 (0,0.5,1,2,5 mg/L) 中.
测量生长曲线,叶绿素a,蛋白质含量和氧化应激标志物 (超氧化物脱酶,催化酶,马隆迪化物).
量化细胞内和细胞外MC-LR水平,并分析光合作用和微囊素相关基因 (psbD1,psaB,rbcL,mcyA,mcyD,mcyH) 的表达.

主要成果:

FE对M. aeruginosa表现出双重作用:刺激0.5-1 mg/L和抑制2-5 mg/L的细胞密度,叶绿素a和蛋白质.
FE暴露增加了氧化应激标志物,并影响了细胞内和细胞外的MC-LR水平.
在更高度下,FE显著抑制了光合作用相关基因和微囊素合成/运输基因 (mcyA,mcyD,mcyH) 的表达.

结论:

氧-p-乙烯对M. aeruginosa生理学,生长和MC-LR产生产生剂量依赖的影响.
FE可以改变M. aeruginosa的氧化应激反应和基因表达模式.
这些发现对于评估FE的生态毒性和促进可持续的除草剂使用至关重要.

关键词:

微囊气球菌的微囊.氧-p-乙基的使用.基因表达基因表达基因表达生理效应是生理效应的影响.毒素毒素是一种毒素.

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