INGEST:一个开源的行为设置,用于研究自我激励的消化行为和学习的操作行为行为.
bioRxiv : the preprint server for biology
|April 1, 2024
概括
研究人员开发了一种INstrument for Gauging Eating and Thirst (INGEST) 仪器,研究大脑如何控制饮食. 这个工具精确地监测消化行为和神经活动,揭示大脑中的Htr3a神经元如何编码这些行为.
科学领域:
- 神经科学是一个神经科学.
- 行为科学 行为科学
- 生理学 生理学 生理学
背景情况:
- 消化行为 (饮食,饮酒) 对于生存至关重要,但经常被孤立地研究,阻碍对它们的综合神经控制的理解.
- 大脑编码内部状态,回忆奖励记忆,激发饮食组合的机制仍然不清楚.
研究的目的:
- 开发一个集成系统,同时高精度测量饮食和饮酒行为.
- 通过使用先进的成像和光遗传学来研究动机性吞行为的神经相关性.
- 探索特定的神经群体如何编码和调节学习和先天的饮食模式.
主要方法:
- 开发INstrument for Gauging Eating and Thirst (INGEST) 测量饮食和口渴的仪器,这是一个定制的行为室,整合了食品/水分配器,仪,摄像头和光遗传学.
- 通过使用Bpod微处理器,同步*in vivo*成像,光遗传学和视频跟踪,并使用精确的行为时间.
- 监测岛内皮质和中部前额皮质 (mPFC) Htr3a神经元活动在各种摄入任务,包括操作学习和选择行为.
主要成果:
- 该INGEsT系统成功实现了详细的消化行为和神经活动的同步记录.
- 发现岛内皮层和mPFC Htr3a神经元的活动在操作学习和选择任务期间编码了摄入行为的多个方面.
- 神经活动模式表现出动物内部状态的调整性,这表明它在调节有动机的行为方面发挥了作用.
结论:
- INGEST平台提供了一个强大的工具,可以精确监测先天和学习的消化行为.
- 特定大脑区域的神经活动,如岛内皮质和mPFC Htr3a神经元,对于编码食用行为至关重要.
- 这项研究为有动机的饮食和饮食行为的神经基础提供了新的见解.
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