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来自Pipistrellus abramus蝙蝠的ACE2是HKU5冠状病毒的受体

Nicholas J Catanzaro¹, Ziyan Wu², Chengcheng Fan²

¹Department of Epidemiology, Gillings School of Global Public Heath, University of North Carolina at Chapel Hill, Chapel Hill, NC, 27599.

bioRxiv : the preprint server for biology

|April 1, 2024

概括

这项研究开发了一种评估merbecovirus细胞进入的方法,揭示了不同的merbecovirus类型使用特定的受体,如DPP4或ACE2侵入宿主细胞. 了解这些进入机制对于评估动物传染病威胁至关重要.

科学领域:

病毒学病毒学
分子生物学分子生物学
结构生物学结构生物学

背景情况:

包括MERS-CoV在内的Merbecoviruses构成动物感染的威胁.
大多数发现的merbecoviruses存在有限的实验室研究.
了解病毒进入是评估传播潜力的关键.

研究的目的:

开发一种可扩展的方法来评估merbecovirus进入细胞的情况.
为了评估整个merbecovirus亚属的受体热带性.
为了调查梅尔贝科病毒进入的结构基础.

主要方法:

开发了一种可扩展的细胞进入测试方法,用于merbecoviruses.
基于尖端糖蛋白的受体结合域 (RBD) 分析的受体使用.
在HKU5尖端蛋白上进行突变分析和冷电子显微镜 (cryo-EM).

主要成果:

梅尔贝科病毒根据受体使用情况被分为分类.
观察到的类特异性:MERS-CoV使用DPP4;其他类,包括HKU5蝙蝠冠状病毒,使用ACE2.
确定了HKU5尖端蛋白中的潜在结构约束,并确定了HKU5-20s尖端剪切剂的冷-EM结构.

结论:

受体热带性是一种在merbecoviruses内保存的,分类特异性的特征.
这项研究提供了关于merbecovirus入境的分子机制的见解.
调查结果有助于评估各种merbecoviruses的动物感染潜力.

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