Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

ER Retrieval Pathway01:45

ER Retrieval Pathway

3.8K
In the secretory pathway, vesicles transport proteins from one cellular compartment to another in forward transport to deliver the protein to its correct location. Occasionally, misfolded proteins and incorrect proteins escape their original compartments, and a retrieval pathway is used to return the escaped proteins to their original compartment.
The ER uses many checkpoints to prevent the entry of incorrectly folded or a resident protein as cargo onto a transport vesicle. These mechanisms...
3.8K
SNAREs and Membrane Fusion01:43

SNAREs and Membrane Fusion

10.9K
Once a transport vesicle has recognized its target organelle, the vesicular membrane needs to fuse with the target membrane to unload the cargo. Transmembrane proteins called SNAREs present on organelle membranes and their vesicles, mediate vesicle fusion.
SNAREs exist in pairs that symmetrically interact and catalyze the fusion of the lipid bilayers in vesicle and target organelle. v-SNARE in the vesicle membrane are single polypeptide chains that bind to a complementary t-SNARE, composed of 2...
10.9K
The Early Endosome: Endocytosis of Transferrin01:28

The Early Endosome: Endocytosis of Transferrin

3.3K
Essential proteins such as insulin or low-density lipoprotein (LDL) and micronutrients such as iron enter a eukaryotic cell through receptor-mediated endocytosis. Subsequently, the early endosomes fuse with the vesicles containing such receptor-ligand complexes and play a vital role in sorting the incoming ligands and receptors. While the ligands are either degraded inside the vesicle or released into the cytosol, their receptors are returned to the plasma membrane for further rounds of...
3.3K
Clathrin Coated Vesicles01:12

Clathrin Coated Vesicles

7.0K
Clathrin-coated vesicles use endocytosis to transport receptors and lysosomal hydrolases from the Golgi to the lysosome in the late secretory pathway. Clathrin-mediated endocytosis was the first described endocytic process, and Clathrin-coated vesicles remain one of the most well-studied transport vesicles. The molecular machinery that generates clathrin-coated vesicles comprises over 50 proteins that precisely coordinate vesicle formation. Cell surface receptors concentrated in indented sites...
7.0K
Tail-anchoring of Proteins in the ER Membrane01:45

Tail-anchoring of Proteins in the ER Membrane

3.1K
Tail-anchored, or TA, proteins are estimated to make up to 3-5% of membrane proteins found in the eukaryotic cell. Such proteins have a single transmembrane domain located approximately 30 amino acid residues upstream from the C-terminal end. As a result, the signal recognition particle (SRP) cannot guide a TA protein to the ER membrane for cotranslational insertion. Hence, they are integrated into the ER membrane post-translationally using their C-terminal end as the anchor. TA proteins...
3.1K
Directing Proteins to the Rough Endoplasmic Reticulum01:34

Directing Proteins to the Rough Endoplasmic Reticulum

7.2K
The organelle-specific signaling sequences direct proteins synthesized in the cytosol to their final destination like ER, mitochondria, peroxisomes, etc. Some of the proteins directed to ER are then trafficked via vesicles to other organelles within the cell or the extracellular environment through the Golgi complex. For example, the rough ER synthesizes soluble proteins for transportation to the lysosomes or secretion out of the cell. It can also synthesize transmembrane proteins that can...
7.2K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Detection and molecular characterization of bovine enterovirus E2 from dairy calves with respiratory disease in Urumqi, Xinjiang, China.

Frontiers in cellular and infection microbiology·2026
Same author

B3GNT7 regulates mucin glycosylation and protects against colitis and infection.

bioRxiv : the preprint server for biology·2026
Same author

PD-1 regulates latent effector differentiation of thymic cytotoxic CD8<sup>+</sup> T cells.

Nature communications·2026
Same author

Association Between Cardiovascular Risk Factors and the Progression of Motor and Non-Motor Symptoms in Parkinson's Disease: A Five-Year Cohort Study.

Journal of clinical medicine·2026
Same author

Green synthesis and slow-release mechanism of biodegradable compound fertilizers.

iScience·2026
Same author

Linkage between HLA-B8 and HLA-DQ2.5 Contributes to Ancestry-Dependent Genetic Risk for Celiac Disease.

medRxiv : the preprint server for health sciences·2026

相关实验视频

Updated: Jun 29, 2025

Expression, Purification, and Liposome Binding of Budding Yeast SNX-BAR Heterodimers
10:28

Expression, Purification, and Liposome Binding of Budding Yeast SNX-BAR Heterodimers

Published on: December 6, 2019

7.8K

恢复者-SNX17组装和内体组分的结构基础.

Amika Singla1, Daniel J Boesch2, Ho Yee Joyce Fung3

  • 1Department of Internal Medicine, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390, USA.

bioRxiv : the preprint server for biology
|April 1, 2024
PubMed
概括

排序Nexin 17 (SNX17) 将货物连接到回收复合体进行内体循环. 我们确定了Retriever-SNX17复杂结构,揭示了影响货物运输和等离子体膜组成的关键相互作用点.

更多相关视频

The Microscopy-Based Assay to Study and Analyze the Recycling Endosomes using SNARE Trafficking
08:51

The Microscopy-Based Assay to Study and Analyze the Recycling Endosomes using SNARE Trafficking

Published on: February 12, 2022

3.5K
In Vitro Polymerization of F-actin on Early Endosomes
12:15

In Vitro Polymerization of F-actin on Early Endosomes

Published on: August 28, 2017

9.0K

相关实验视频

Last Updated: Jun 29, 2025

Expression, Purification, and Liposome Binding of Budding Yeast SNX-BAR Heterodimers
10:28

Expression, Purification, and Liposome Binding of Budding Yeast SNX-BAR Heterodimers

Published on: December 6, 2019

7.8K
The Microscopy-Based Assay to Study and Analyze the Recycling Endosomes using SNARE Trafficking
08:51

The Microscopy-Based Assay to Study and Analyze the Recycling Endosomes using SNARE Trafficking

Published on: February 12, 2022

3.5K
In Vitro Polymerization of F-actin on Early Endosomes
12:15

In Vitro Polymerization of F-actin on Early Endosomes

Published on: August 28, 2017

9.0K

科学领域:

  • 细胞生物学 细胞生物学
  • 结构生物学 结构生物学
  • 分子机制的分子机制

背景情况:

  • 排序Nexin 17 (SNX17) 对于内体循环至关重要,它通过Retriever复合体调解载荷蛋白质的运输.
  • 控制SNX17-Retriever相互作用的精确分子机制在很大程度上是未知的.

研究的目的:

  • 阐明Sorting Nexin 17 (SNX17) 与Retriever复合体之间的相互作用的结构基础.
  • 了解这种相互作用如何影响内体体贩运和货物蛋白回收.

主要方法:

  • 低温电子显微镜 (cryo-EM) 用于确定复杂的结构.
  • 生物化学测试,细胞研究和蛋白质组分析以验证功能影响.

主要成果:

  • SNX17的C终端尾部与检索器复合体 (VPS35L/VPS26C子单元) 上的保留接口结合.
  • 这种接口的破坏会损害SNX17-Retriever的相互作用,影响SNX17依赖的货物循环和血膜蛋白质组合.
  • 在Retriever上,SNX17结合部位容纳了具有类似酸性C终端图案的其他配体.

结论:

  • 对SNX17-Retriever相互作用的结构和功能洞察力为内体贩运提供了一个基本的机制.
  • 检索器综合体充当一个枢纽,通过保留的结合动机与多个调节因素潜在地相互作用.