Search research articles

关于 JoVE

概览领导团队博客 JoVE 帮助中心

作者

出版流程编辑委员会范围与政策同行评审常见问题投稿

图书馆员

用户评价订阅访问资源图书馆顾问委员会常见问题

研究

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments 存档

教育

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual 教师资源中心教师网站

使用条款与条件

相关概念视频

Three-Dimensional Microscopy in Microbiology

Three-Dimensional Microscopy in Microbiology

Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序

Same author

SPRM: spatial process and relationship modeling for multiplexed images.

Bioinformatics advances·2026

Same author

CytoSpatio: Learning cell type spatial relationships using multirange, multitype point process models.

PLoS computational biology·2025

Same author

Big1 is a cell cycle regulator linking cell size to basal body number.

bioRxiv : the preprint server for biology·2025

Same author

CytoSpatio: Learning cell type spatial relationships using multirange, multitype point process models.

bioRxiv : the preprint server for biology·2024

Same author

Improved protein interaction models predict differences in complexes between human cell lines.

bioRxiv : the preprint server for biology·2024

Same author

Flexible and robust cell type annotation for highly multiplexed tissue images.

bioRxiv : the preprint server for biology·2024

Same journal

A human-specific genetic modifier reconfigures large-scale cortical network dynamics underlying behavioral performance.

bioRxiv : the preprint server for biology·2026

Same journal

<i>Staphylococcus aureus</i> uses a eukaryotic-like uridyltransferase to make UDP-GlcNAc for cell wall synthesis.

bioRxiv : the preprint server for biology·2026

Same journal

Dynamic redistribution of eIF4F controls cap-dependent translation initiation.

bioRxiv : the preprint server for biology·2026

Same journal

When does additional information improve accuracy of RNA secondary structure prediction?

bioRxiv : the preprint server for biology·2026

Same journal

Normative brain-state trajectories reveal deviation from healthy aging in Alzheimer's disease.

bioRxiv : the preprint server for biology·2026

Same journal

Noradrenergic infraslow rhythm during sleep is the critical link between heart-rate dynamics and memory consolidation.

bioRxiv : the preprint server for biology·2026

查看所有相关文章

Search research articles

相关实验视频

Updated: Jun 18, 2026

From Voxels to Knowledge: A Practical Guide to the Segmentation of Complex Electron Microscopy 3D-Data

From Voxels to Knowledge: A Practical Guide to the Segmentation of Complex Electron Microscopy 3D-Data

Published on: August 13, 2014

3DCellComposer - - 一个使用二维细胞分割方法进行三维细胞分割的多功能管道.

Haoran Chen¹, Robert F Murphy¹

¹Computational Biology Department, School of Computer Science, Carnegie Mellon University, 5000 Forbes Avenue, Pittsburgh PA 15213, USA.

bioRxiv : the preprint server for biology

|April 1, 2024

概括

此摘要是机器生成的。

本研究介绍了3DCellComposer,这是一个开源工具,可以使用现有的2D模型进行准确的3D细胞细分. 它克服了数据的局限性,并增强了大规模项目的生物图像分析.

关键词:

三维显微镜的3D显微镜细胞细分细胞细分细胞细分图像成像质谱学质谱学多重光成像多重光成像核细分的核细分是指核细分.空间蛋白质组学空间蛋白质组学组织图像组织图像

更多相关视频

Correlative Confocal and 3D Electron Microscopy of a Specific Sensory Cell

Correlative Confocal and 3D Electron Microscopy of a Specific Sensory Cell

Published on: July 19, 2015

A Method for 3D Reconstruction and Virtual Reality Analysis of Glial and Neuronal Cells

A Method for 3D Reconstruction and Virtual Reality Analysis of Glial and Neuronal Cells

Published on: September 28, 2019

相关实验视频

Last Updated: Jun 18, 2026

From Voxels to Knowledge: A Practical Guide to the Segmentation of Complex Electron Microscopy 3D-Data

From Voxels to Knowledge: A Practical Guide to the Segmentation of Complex Electron Microscopy 3D-Data

Published on: August 13, 2014

Correlative Confocal and 3D Electron Microscopy of a Specific Sensory Cell

Correlative Confocal and 3D Electron Microscopy of a Specific Sensory Cell

Published on: July 19, 2015

A Method for 3D Reconstruction and Virtual Reality Analysis of Glial and Neuronal Cells

A Method for 3D Reconstruction and Virtual Reality Analysis of Glial and Neuronal Cells

Published on: September 28, 2019

科学领域:

生物图像信息学信息学
计算生物学计算生物学
细胞成像细胞成像

背景情况:

精确的细胞细分对于生物图像信息学中的细胞分析至关重要.
由于数据稀缺性和复杂性,3D细胞细分比2D方法开发较少.
现有的3D方法往往缺乏跨不同成像模式的概括性.

研究的目的:

使用2D方法开发一种可通用的方法,用于使用精确的3D细胞细分.
介绍3DCellComposer,这是一个开源软件包,用于多功能3D细胞细分.
为了提高3D细分的质量评估,而不是人类注释.

主要方法:

在3DCellComposer包中实施了一种新的方法.
使用户能够集成现有的2D细胞细分模型.
增强的细胞细分评估器用于3D图像质量评估.

主要成果:

使用开发的方法证明了组织图像的高质量3D细分.
与现有的3D方法相比,在细胞培养图像上展示了更高的性能.
验证了该方法在不同成像模式中的通用性.

结论:

3DCellComposer为手动3D注释和模型培训提供了一个有价值的替代方案.
该工具有助于大型生物成像项目,如人类生物分子地图计划.
它减少了对新样本类型的广泛数据采集和培训的需求.